Mehdi Ghorbal

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Genome manipulation in the malaria parasite Plasmodium falciparum remains largely intractable and improved genomic tools are needed to further understand pathogenesis and drug resistance. We demonstrated the CRISPR-Cas9 system for use in P. falciparum by disrupting chromosomal loci and generating marker-free, single-nucleotide substitutions with high(More)
Artemisinins are the cornerstone of anti-malarial drugs. Emergence and spread of resistance to them raises risk of wiping out recent gains achieved in reducing worldwide malaria burden and threatens future malaria control and elimination on a global level. Genome-wide association studies (GWAS) have revealed parasite genetic loci associated with artemisinin(More)
Protozoan pathogens that cause leishmaniasis in humans are relatively refractory to genetic manipulation. In this work, we implemented the CRISPR-Cas9 system in Leishmania parasites and demonstrated its efficient use for genome editing. The Cas9 endonuclease was expressed under the control of the Dihydrofolate Reductase-Thymidylate Synthase (DHFR-TS)(More)
We report 4 cases of gelatin drop dystrophy corneal amyloidosis in two brothers and two sisters of the same family. The age of onset is between 1 and 10 years. The corneal signs are described at different stages of development. Lesions are initially asymmetrical. The typical is a subepithelial nodule starting in the center and then expanding to involve the(More)
Antiphospholipid antibodies may play and important role in the pathogenesis of retinal vascular occlusions; investigated the prevalence among 33 patients with retinal vein and artery occlusions and 80 controls. Prevalence was 33% and 5% respectively. Ophthalmic examination and fluorescein angiography showed that occlusions were due to ischaemic events. The(More)
Measles was the second leading cause of infant mortality in Tunisia prior to introduction of measles vaccination in 1979. The number of reported measles cases has decreased from 3007 in 1981 to 47 cases in 2000 due in part to the high coverage rates achieved after 1992. During 1998, a measles catch-up campaign vaccinated 1,846,657 children (95%) aged 6-16(More)
Plasmodium falciparum virulence is linked to its ability to sequester in post-capillary venules in the human host. Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is the main variant surface antigen implicated in this process. Complete loss of parasite adhesion is linked to a large subtelomeric deletion on chromosome 9 in a number of(More)
Records of 2180 tubal sterilizations carried out using the Yoon procedure between March 1978-March 1980 in a pilot family planning clinic in Tunis, Tunisia, were examined to assess the medical, social, and psychosexual impact of the operation. Patients were seen by a social worker who obtained the couple's consent and evaluated their socioeconomic status,(More)
1Higher Institute of Biotechnology, Monastir, Tunisia. 2Department of Ophthalmology, Farhat Hached University Hospital, Sousse, Tunisia. Haematology and Autoimmune Diseases Unit, Faculty of Pharmacy, University of Monastir, Tunisia. Received: 29/04/03; accepted: 23/12/03 ABSTRACT Antiphospholipid antibodies may play an important role in the pathogenesis of(More)