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We employed gene targeting to study H2AX, a histone variant phosphorylated in chromatin surrounding DNA double-strand breaks. Mice deficient for both H2AX and p53 (H(delta/delta)P(-/-)) rapidly developed immature T and B lymphomas and solid tumors. Moreover, H2AX haploinsufficiency caused genomic instability in normal cells and, on a p53-deficient(More)
Antibody class switching in activated B cells uses class switch recombination (CSR), which joins activation-induced cytidine deaminase (AID)-dependent double-strand breaks (DSBs) within two large immunoglobulin heavy chain (IgH) locus switch (S) regions that lie up to 200 kilobases apart. To test postulated roles of S regions and AID in CSR, we generated(More)
HR factors in mice causes increased ionizing radiation (IR) sensitivity, genomic instability, and embryonic le-thality, while in humans, such mutations have been * sensitivity, genomic instability, severely impaired lym-1 Howard Hughes Medical Institute phocyte development, and dramatically increased lym-The Children's Hospital phoma predisposition in the(More)
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