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Although it is widely regarded that the targeting of RNA molecules to subcellular destinations depends upon the recognition of cis-elements found within their 3' untranslated regions (UTR), relatively little is known about the specific features of these cis-sequences that underlie their function. Interaction between specific repeated motifs within the 3'(More)
The Bacillus subtilis proteins DnaD and DnaB are essential for replication initiation and are conserved in low G+C content Gram-positive bacteria. Previous work indicated that DnaD and DnaB are involved in helicase loading during the process of restarting stalled replication forks. We have investigated the roles of DnaD and DnaB in replication initiation at(More)
The DNA replication machinery, various regions of the chromosome, and some plasmids occupy characteristic subcellular positions in bacterial cells. We visualized the location of a multicopy plasmid, pHP13, in living cells of Bacillus subtilis using an array of lac operators and LacI-green fluorescent protein (GFP). In the majority of cells, plasmids(More)
BACKGROUND The Bacillus subtilis genes dnaD and dnaB are essential for the initiation of DNA replication and are required for loading of the replicative helicase at the chromosomal origin of replication oriC. Wild type DnaD and DnaB interact weakly in vitro and this interaction has not been detected in vivo or in yeast two-hybrid assays. (More)
Teaching about proteins, how their structure is specified, and how in turn this influences their function has become an integral curriculum component for both introductory biology and biochemistry undergraduate courses. To understand how proteins work, students first learn about protein structure and its relationship to protein function. StarBiochem is a(More)
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story matters. Abstract Background: The Bacillus subtilis genes dnaD and dnaB are essential(More)
Despite attempts to increase enrollment of under-represented minorities (URMs: primarily Black/African American, Hispanic/Latino, and Native American students) in health professional programs, limited progress has been made. Compelling reasons to rectify this situation include equity for URMs, better prepared health professionals when programs are diverse,(More)
Origin of exocrine pancreatic cells from nestin-positive precursors in developing mouse pancreas 15 Ce-Y14 and MAG-1, components of the exon–exon junction complex, are required for embryogenesis and germline sexual switching in Caenorhabditis elegans 27 The grainy head transcription factor is essential for the function of the frizzled pathway in the(More)
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