Megan A. Mayo

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Northern blot analysis of nucleic acid from potato plant tissues and tobacco protoplasts infected with a Scottish isolate of potato leafroll luteovirus (PLRV) detected the 6 kb genomic RNA and one subgenomic RNA species of about 2.7 kb; RNA extracted from virus particles contained only the genomic species. Blotting with small defined probes suggested that(More)
The discrepancy between published sequences of the 5' non-coding regions of RNA of a Scottish (S) and that of Dutch (D), Australian and Canadian isolates of potato leafroll virus (PLRV) was investigated. Reverse transcription followed by amplification by polymerase chain reaction showed that RNA from three distinct Scottish isolates of PLRV contained(More)
  • Christiane Fritsch, M A Mayo, And A F Murant
  • 1979
SUMMARY In wheat germ extracts and reticulocyte lysates the genome RNA molecules of tomato black ring virus (TBRV), RNA-I (tool. wt. 2.8 × lO 6) and RNA-2 (mol. wt. 1.6 × IO6), were translated into products of maximum mol. wt. 2.2 × lO 5 and 1.6 × lO 5, respectively. These products represent about 8o % and IOO % of the coding capacity of the two RNA(More)
In plants infected with Potato leafroll virus (PLRV), or other luteoviruses, infection is very largely confined to cells in the vascular system. Even in tobacco plants transformed with PLRV full-length cDNA, in which all mesophyll cells should synthesize infectious PLRV RNA transcripts, only a minority of the mesophyll cells accumulate detectable amounts of(More)
A full-length cDNA copy of the genome of Potato leafroll virus (PLRV) was introduced into the genome of tobacco and potato plants by Agrobacterium tumefaciens-mediated transformation. Transgenic lines were obtained in which the transgene was readily detected by PCR with DNA extracted from T(1) tobacco seedlings and clonally multiplied potato plants.(More)
Raspberry bushy dwarf virus (RBDV) has isometric, 33 nm diameter particles and a bipartite RNA genome. Sequencing of the larger component (RNA-1) showed that it consists of 5449 nucleotides and contains one large open reading frame encoding a putative translation product with a calculated M(r) of 190,000. Comparisons of this polypeptide with non-structural(More)
SUMMARY Translation of the satellite RNA (RNA-3) of tomato black ring virus (TBRV) in wheat germ extracts or reticulocyte lysates resulted in the synthesis of a poly-peptide of mol. wt. about 48 ooo, both in the presence and in the absence of RNA-I and RNA-2. The RNA-3 specific polypeptide of TBRV strain G was slightly larger than that of strain S. A(More)
The complete sequence of 9871 nucleotides (nts) of parsnip yellow fleck virus (PYFV; isolate P-121) was determined from cDNA clones and by direct sequencing of viral RNA. The RNA contains a large open reading frame between nts 279 and 9362 which encodes a polyprotein of 3027 amino acids with a calculated M(r) of 336212 (336K). A PYFV polyclonal antiserum(More)
Just a few years ago, genomic studies of non-model organisms were extremely time and cost prohibitive, but recent advances in next-generation sequencing have made it possible to generate hundreds to thousands of informative single nucleotide polymorphisms (SNPs) for non-model organisms. We generated a SNP panel using restriction site-associated DNA (RAD)(More)
A full-length cDNA corresponding to the RNA genome of Potato leafroll virus (PLRV) was modified by inserting cDNA that encoded the jellyfish green fluorescent protein (GFP) into the P5 gene near its 3' end. Nicotiana benthamiana protoplasts electroporated with plasmid DNA containing this cDNA behind the 35S RNA promoter of Cauliflower mosaic virus became(More)
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