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Sensors for the quantification, localization and analysis of the dynamics of plant hormones
- R. Isoda, Akira Yoshinari, Masayoshi Nakamura
- BiologyThe Plant journal : for cell and molecular…
- 24 November 2020
The diverse set of tools available for quantifying and visualizing hormone distribution and dynamics, including transcriptional reporters, degradation sensors, and luciferase and fluorescent sensors, are summarized.
Differential regulation of glucose transport activity in yeast by specific cAMP signatures.
- C. Bermejo, F. Haerizadeh, Mayuri Sadoine, Diane Chermak, W. Frommer
- BiologyThe Biochemical journal
- 15 June 2013
A novel function of the Gpr1/cAMP/PKA pathway was identified in glucose-starved cells: during starvation the GPR1/ cAMP/ PKA pathway is required to maintain Hxt5p activity in the absence of glucose-induced cAMP spiking.
Selective Double-Labeling of Cell-Free Synthesized Proteins for More Accurate smFRET Studies.
- Mayuri Sadoine, M. Cerminara, Noémie Kempf, M. Gerrits, J. Fitter, Alexandros Katranidis
- BiologyAnalytical chemistry
- 25 October 2017
This work presents an approach of cell-free protein synthesis (CFPS) that provides proteins with two different functional groups for post-translational labeling at the specific amino acid positions, and achieves not only a site-specific but, most importantly, also a sites-selective, label scheme that permits the highest accuracy of measured data.
Affinity Series of Genetically Encoded Förster Resonance Energy-Transfer Sensors for Sucrose.
A two-step approach to first improve the dynamic range of the FLIPsuc sensor and then expand the detection range from micro- to millimolar sucrose concentrations by mutating a key residue in the binding site may enable investigation of sucrose transporter candidates and comprehensive in vivo analyses of Sucrose concentration in plants.
Cotranslational Incorporation into Proteins of a Fluorophore Suitable for smFRET Studies.
- Mayuri Sadoine, M. Cerminara, M. Gerrits, J. Fitter, Alexandros Katranidis
- Biology, ChemistryACS synthetic biology
- 31 January 2018
It is shown that cotranslational incorporation of good fluorophores into proteins is a viable strategy to produce suitable samples for smFRET studies.
Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology
A brief history of the development of genetically encoded sensors and an overview of the types of sensors available for quantifying and visualizing ion and metabolite distribution and dynamics are provided.
Preparation of Cell-free Synthesized Proteins Selectively Double Labeled for Single-molecule FRET Studies.
- Mayuri Sadoine, J. Fitter, Alexandros Katranidis, M. Cerminara
- Biology, ChemistryBio-protocol
- 20 June 2018
The protocol describes two cell-free approaches to achieve a selective label scheme that allows the highest possible accuracy in inter-dye distance determination.
OzTracs: Optical Osmolality Reporters Engineered from Mechanosensitive Ion Channels
Genetically encoded osmolality sensors (OzTracs) are engineered by fusing green fluorescent protein spectral variants to the mechanosensitive ion channels MscL from E. coli or MSL10 from A. thaliana to serve as osmlality-dependent reporters through an indirect mechanism, such as effects on molecular crowding or fluorophore solvation.
Sucrose-dependence of sugar uptake, quorum sensing and virulence of the rice blight pathogen Xanthomonas oryzae pv. oryzae
- Juying Long, Mayuri Sadoine, Congfeng Song, Yugander Arra, W. Frommer, Bing Yang
- 22 August 2021
Evidence is provided that both the ability to attack as well as the growth of bacteria depend on sucrose uptake, and a regulatory network including transcriptional regulators, quorum sensing, swimming, biofilm production and virulence that all depend on Sucrose uptake is unraveled.