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Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation
While the catalog of mammalian transcripts and their expression levels in different cell types and disease states is rapidly expanding, our understanding of transcript function lags behind. WeExpand
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CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs.
Developing technologies for efficient and scalable disruption of gene expression will provide powerful tools for studying gene function, developmental pathways, and disease mechanisms. Here, weExpand
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A Systematic Mammalian Genetic Interaction Map Reveals Pathways Underlying Ricin Susceptibility
Genetic interaction (GI) maps, comprising pairwise measures of how strongly the function of one gene depends on the presence of a second, have enabled the systematic exploration of gene function inExpand
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Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation
We recently found that nucleosomes directly block access of CRISPR/Cas9 to DNA (Horlbeck et al., 2016). Here, we build on this observation with a comprehensive algorithm that incorporates chromatin,Expand
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CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells
A very focused function for lncRNAs The human genome generates many thousands of long noncoding RNAs (lncRNAs). A very small number of lncRNAs have been shown to be functional. Liu et al. carried outExpand
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A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response
Functional genomics efforts face tradeoffs between number of perturbations examined and complexity of phenotypes measured. We bridge this gap with Perturb-seq, which combines droplet-basedExpand
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Single-cell analysis of long non-coding RNAs in the developing human neocortex
BackgroundLong non-coding RNAs (lncRNAs) comprise a diverse class of transcripts that can regulate molecular and cellular processes in brain development and disease. LncRNAs exhibit cell type- andExpand
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Nucleosomes impede Cas9 access to DNA in vivo and in vitro
The prokaryotic CRISPR (clustered regularly interspaced palindromic repeats)-associated protein, Cas9, has been widely adopted as a tool for editing, imaging, and regulating eukaryotic genomes.Expand
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ER Cargo Properties Specify a Requirement for COPII Coat Rigidity Mediated by Sec13p
A Fair COP During eukaryotic intracellular membrane traffic, how is membrane curvature imparted by the cytoplasmic proteins that form the COPII coat, which mediates vesicle budding from theExpand
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Mapping the Genetic Landscape of Human Cells
Seminal yeast studies have established the value of comprehensively mapping genetic interactions (GIs) for inferring gene function. Efforts in human cells using focused gene sets underscore theExpand
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