Paolo Ascenzi7
Gabriella Fanali7
Leonardo Lopiano7
Massimo Coletta6
7Paolo Ascenzi
7Gabriella Fanali
7Leonardo Lopiano
6Massimo Coletta
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Dopaminergic human neuroblastoma SH-SY5Y cells were stably transformed to increase expression of alpha-synuclein, a Parkinson's disease-related protein. Transformed cells were more resistant to oxidative insults, showing a cytoprotective role of alpha-synuclein. The expression of redox chaperonins (DJ-1, HSP70, and 14-3-3) was evaluated by Western blotting.(More)
Parkinson's disease is a common neurodegenerative disorder with a mainly sporadic aetiology, although a number of monogenic familiar forms are known. Most of the motor symptoms are due to selective depletion of dopaminergic, neuromelanin-containing neurones of the substantia nigra pars compacta. Neuromelanin is the dark insoluble macromolecule that confers(More)
MOTIVATION The two-dimensional electrophoresis (2-DE) pattern of proteins is thought to be specifically related to the physiological or pathological condition at the moment of sample preparation. On this ground, most proteomic studies move to identify specific hallmarks for a number of different conditions. However, the information arising from these(More)
The pigmentation of substantia nigra pars compacta dopaminergic neurons is due to the presence of neuromelanin, an irregular macromolecular pigment belonging to the family of melanins. Depletion of neuromelanin in Parkinson's disease is typically indicated by loss of brown color in this area. Unlike that from controls, the pigment extracted from substantia(More)
  • Marco Bisaglia, Isabella Tessari, Luca Pinato, Massimo Bellanda, Sabrina Giraudo, Mauro Fasano +3 others
  • 2005
Human alpha-synuclein is a 140-amino acid protein of unknown function abundantly expressed in the brain and found in Lewy bodies, a characteristic feature of Parkinson's disease. Alpha-synuclein is random in water under physiological conditions, but the first approximately 100 residues interact with SDS micelles or acidic phospholipid small unilamellar(More)
  • Paolo Ascenzi, Andrea Coletta, Yu Cao, Viviana Trezza, Loris Leboffe, Gabriella Fanali +5 others
  • 2013
Isoniazid represents a first-line anti-tuberculosis medication in prevention and treatment. This prodrug is activated by a mycobacterial catalase-peroxidase enzyme called KatG in Mycobacterium tuberculosis), thereby inhibiting the synthesis of mycolic acid, required for the mycobacterial cell wall. Moreover, isoniazid activation by KatG produces some(More)
Human serum heme–albumin (HSA–heme–Fe) displays reactivity and spectroscopic properties similar to those of heme proteins. Here, the nitrite reductase activity of ferrous HSA–heme–Fe [HSA–heme–Fe(II)] is reported. The value of the second-order rate constant for the reduction of $$ {\text{NO}}_{2}^{ - } $$ NO 2 - to NO and the concomitant formation of(More)
  • Giuliano Dall 'o, Valentina Belli, Mauro Brolis, Ivan Mozzi, Mauro Fasano
  • 2013
The topic of nearly zero-energy buildings (n-ZEB), introduced by the Directive 2010/31/EU will direct the building market toward ever greater energy efficiency of new buildings. In some contexts, however, the building market for high-efficiency buildings has evolved, in recent years, on the basis of national and regional laws that have contributed to the(More)
In this review, we report how proteomic methodologies have been used to investigate cellular and animal models of Parkinson's disease (PD), with a special focus on alpha-synuclein. PD is a complex, multifactorial neurodegenerative disease affecting approximately 2% of the population over 65 years of age, pathologically characterized by alpha-synuclein(More)
  • Alessio Bocedi, Giampiero De Sanctis, Chiara Ciaccio, Grazia R. Tundo, Alessandra Di Masi, Gabriella Fanali +5 others
  • 2013
Human serum albumin (HSA), the most abundant protein in human plasma, could be considered as a prototypic monomeric allosteric protein, since the ligand-dependent conformational adaptability of HSA spreads beyond the immediate proximity of the binding site(s). As a matter of fact, HSA is a major transport protein in the bloodstream and the regulation of the(More)