Over the past decades, plant cryopreservation techn ologies have been evolving rapidly, opening the door to the possibility of longterm storage of valuable genetic resources of many crop and forest… (More)
 Shoot tips from in vitro-grown, cold-hardened stock plants of white poplar (Populus alba L.) were successfully cryopreserved at –196  °C by one-step vitrification. After preculturing at 5  °C for 2… (More)
Adventitious buds of Cupressus sempervirens L., were formed on excised mature embryos cultured for 10 days on half-strength Quoirin and Lepoivre medium (1/2QP) with 10 μM N6-benzyladenine. For shoot… (More)
1 Key Laboratory of Plant Genetic Improvement of Northwest China, College of Horticulture, Northwest Agricultural and Forest University, Yangling, Shaanxi, China 2 Department of Applied Biology,… (More)
Studies were undertaken to optimize tissue culture conditions for micropropagation of Aleppo pine (Pinus halepensis Mill.) from mature embryos and various explants of the embryo. Over 90% of the… (More)
Today, the conservation of ornamental germplasm can take advantage of innovative techniques which allow preservation in vitro (slow growth storage) or in liquid nitrogen (cryopreservation) of plant… (More)
Cryopreservation protocols by dehydration and one-step freezing were developed for seeds from three Pistacia species, i.e., P. vera, P. terebinthus and P. lentiscus, which were characterised by… (More)
Since its development in the 1960s, plant cryopreservation is considered an extraordinary method of safe long-term conservation of biological material, as it does not induce genetic alterations and… (More)
The effectiveness of a protocol for somatic embryogenesis in conifers requires both the proliferation of embryonal masses and their conversion into somatic plants. Despite several successful… (More)
This study was carried out on Kober 5BB (Vitis Berlandieri × V. riparia) grape rootstock shoot tips during the preparatory steps preceding the direct immersion in liquid nitrogen, in order to… (More)