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A polyphasic approach has been developed to gain knowledge of suitable key indicators for the evaluation of environmental impact of genetically modified Bt 11 and Bt 176 corn lines on soil ecosystems. We assessed the effects of Bt corn (which constitutively expresses the insecticidal toxin from Bacillus thuringiensis, encoded by the truncated Cry1Ab gene)(More)
We performed a longitudinal study (repeated observations of the same sample over time) to investigate both the composition and structure of temporal changes of bacterial community composition in soil mesocosms, subjected to three different treatments (water and 5 or 25 mg kg−1 of dried soil Cd2+). By analogy with the pan genome concept, we identified a core(More)
The use of microorganisms as bioindicators of soil health is quite a new feature, rarely considered for the soil health evaluation in chronically-polluted industrial sites, and still suffering of the bias related to the technique applied. In this work we applied a microbiological polyphasic approach, relying on soil indigenous microorganisms as(More)
Restriction analysis of amplified 16S rDNA from bacterial strains isolated from soil was used to evaluate the effects of swine manure and urea fertilisation on indigenous populations of the plant growth promoting bacterium, Azospirillum brasilense, in corn-cultivated experimental plots. Several operational taxonomic units (OTUs) were found among the(More)
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