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Programmable Ligand Detection System in Plants through a Synthetic Signal Transduction Pathway
TLDR
This work describes assembly and function of a complete synthetic signal transduction pathway in plants that links input from computationally re-designed periplasmic binding proteins to a visual response and may allow plants to serve as a simple and inexpensive means to monitor human surroundings for substances such as pollutants, explosives, or chemical agents.
Engineering key components in a synthetic eukaryotic signal transduction pathway
TLDR
It is shown that sequence conservation and cross talk can extend across kingdoms and can be exploited to produce a synthetic plant signal transduction system.
Computational design of environmental sensors for the potent opioid fentanyl
TLDR
The computational design of proteins that bind the potent analgesic fentanyl is described, using the designs to generate plant sensors for fentanyl by coupling ligand binding to design stability and should be generally useful for detecting toxic hydrophobic compounds in the environment.
A general strategy to construct small molecule biosensors in eukaryotes
TLDR
The biosensors based on a ligand-binding domain (LBD) are produced by using a method that, in principle, can be applied to any target molecule and used to improve the biotransformation of pregnenolone to progesterone in yeast and to regulate CRISPR activity in mammalian cells.
Targeted DNA excision in Arabidopsis by a re-engineered homing endonuclease
BackgroundA systematic method for plant genome manipulation is a major aim of plant biotechnology. One approach to achieving this involves producing a double-strand DNA break at a genomic target site
RAPD ANALYSIS OF CALLUS REGENERATED AND SEED GROWN PLANTS OF MAIZE (Zea mays L.)
TLDR
The results indicate that RAPD technique can be used as a potent and easy-to-use tool to identify maize inbred lines less susceptible to somaclonal variation when cultured in vitro, a fundamental step in plant genetic transformation.
Quantitative characterization of genetic parts and circuits for plant synthetic biology
TLDR
A method for rapid quantitative characterization of genetic plant parts using transient expression in protoplasts and dual luciferase outputs is described, which enables the construction of tunable gene circuits in complex eukaryotic organisms.
A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity.
TLDR
A synthetic de-greening circuit is designed that produced rapid chlorophyll loss on perception of a specific input, providing the first easily re- settable reporter system for plants and the capacity to make re-settable biosensors.
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