Maurice Weckx

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An alcohol dehydrogenase of broad specificity was purified 43-fold from extracts of Mycobacterium tuberculosis var. bovis (BCG) grown on Sauton medium. It was also present in cells grown in Dubos medium and Tween 80 and bovine serum albumin. The enzyme, which appeared to be soluble, acted as an oxidoreductase in the system butan-1-ol-NADP. It was eluted(More)
An immunogenic protein called P32 has been purified from Sauton zinc deficient culture filtrate of Mycobacterium bovis BCG using successively hydrophobic chromatography on Phenyl-Sepharose, ion exchange on DEAE-Sephacel and molecular sieving on Sephadex G-100. The final preparation was found to be homogeneous as based on several analyses. This P32 protein(More)
  • J De Bruyn, Robert Bosmans, +6 authors M Harboe
  • Infection and immunity
  • 1987
An immunogenic and skin-reactive protein called P64 was purified from Sauton zinc-deficient culture filtrate of Mycobacterium bovis BCG by using successively hydrophobic chromatography on phenyl-Sepharose, ion exchange on DEAE-Sephacel, and molecular sieving on Sephadex G-200. The final P64 preparation was found to be homogeneous based on several analyses.(More)
The ATP content and oxygen uptake rate, two parameters of viability of a BCG suspension, are compared. The lack of correlation between measurements made on fresh routine preparations indicates that the ATP measurements is affected by the degree of dispersion of the preparation. Experimental preparations made under standard conditions (constant semi-dry(More)
Mycobacterium bovis strain BCG, substrain 1173P2, has been grown in homogeneous culture in classical synthetic Sauton medium without supplementary ingredients. The culture conditions are described. The protein release in the culture medium and the tuberculin yield after 2% trichloroacetic acid precipitation were significantly improved. The antigenicity of(More)
Transsphenoi dal surgery for sellar region tumors is traditionally done only by neurosurgeons. The use of endoscopes has permitted a direct transnasal approach to the sphenoidal sinus, without dissection of the septal mucosa, reducing postoperative morbidity. Aim: The purpose of this study was to assess the technical difficulties, and per and postoperative(More)
Mycobacterium tuberculosis var. bovis (BCG) grown on Sauton medium normally forms a pellicle; in the absence of added Zn2+, however, the pellicle sank during incubation and the yield was only about 20% of normal. The Zn2+-starved bacteria were morphologically similar to normal bacteria and were still acid-fast at 7 d as well as 14 d. The Zn2+-starved(More)
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