Matthias Meier

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Here, we present the full integration of a proximity ligation assay (PLA) on a microfluidic chip for systematic cell signaling studies. PLA is an in situ technology for the detection of protein interaction, post-translational modification, concentration, and cellular location with single-molecule resolution. Analytical performance advances on chip are(More)
Configurable hardware is becoming increasingly powerful and less expensive. This allows embedded system developers to exploit hardware parallelism in order to improve real time properties and energy efficiency. However, hardware design, even if performed using high-level hardware description languages, is error-prone and time consuming, especially when(More)
Recent gut microbiome studies in model organisms emphasize the effects of intrinsic and extrinsic factors on the variation of the bacterial composition and its impact on the overall health status of the host. Species occurring in the same habitat might share a similar microbiome, especially if they overlap in ecological and behavioral traits. So far, the(More)
Assembly cloning is increasingly replacing conventional restriction enzyme and DNA-ligase-dependent cloning methods for reasons of efficiency and performance. Here, we describe AQUA (advanced quick assembly), a simple and versatile seamless assembly cloning approach. We demonstrate the applicability and versatility of AQUA Cloning in selected(More)
In the present study, we developed a microfluidic large-scale integration (mLSI) platform for the temporal and chemical control of cell cultures to study fast kinetics of protein phosphorylation. For in situ protein analysis the mLSI chip integrates the Proximity Ligation Assay (PLA). To investigate cell-signaling events with a time resolution of a few(More)
Hardware description languages are a promising field for the application of aspect technology. In a case study with the MB-Lite soft core CPU, which is an open, cycle accurate re-implementation of Xilinx' Microblaze processor, we show that crosscutting concerns in hardware structures actually exist. After discussing the semantic differences between(More)
Transactional memory is regarded as a very promising technology to deal with concurrency control in future multicore and manycore systems. While a lot of software, hardware, and hybrid transactional memory implementations have been proposed and analyzed, the silver bullet still hasn't been found. The main reason is that the performance of transactional(More)
Fluorogenic RNAs that are based on the complex formed by 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) derivatives and the RNA aptamer named Spinach were used to engineer a new generation of in vitro and in vivo sensors for bioanalytics. With the resolved crystal structure of the RNA/small molecule complex, the engineering map becomes available,(More)