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Axoplasmic proteins containing nuclear localization signals (NLS) signal retrogradely by an unknown mechanism in injured nerve. Here we demonstrate that the importin/karyopherin alpha and beta families underlie this process. We show that importins are found in axons at significant distances from the cell body and that importin beta protein is increased(More)
Proteins actively transported into the nucleus via the classical nuclear import pathway contain nuclear localization signals (NLSs), which are recognized by the family of importin alpha molecules. Importin alpha contains 10 armadillo (arm) repeats, of which the N-terminal arm repeats 2-4 have been considered as the "major" NLS binding site.(More)
In this paper, viable applications for mobile robotic units on construction sites are explored. While identifying potential areas for in-situ fabrication in the construction sector, the intention is also to build upon innovative man-machine interaction paradigms to deal with the imprecision and tolerances often faced on construction sites. By combining the(More)
That matter is secondary to shape constitutes the fallacy of design after craft. By nature, and in its rite, the material practice of craft is informed by matter, its method of fabrication, and by the environment (Semper, 1851). As in Nature, when creation begins with matter, morphogenesis, or the generation of form, is a process engendered by the physical(More)
In order to contribute to the understanding of the activation of the oncoprotein p53 we determined the metabolic stability of p53 in a variety of non-transformed, immortalized and SV40- and non-SV40-transformed cell lines. In addition, we analyzed the metabolic stability of the SV40 large T antigen in SV40 transformed cell lines. Pulse-chase experiments(More)
The "classical" nuclear import pathway depends on importins alpha and beta. Humans have only one importin beta, while six alpha importins have been described. Whether or not distinct alpha importins are essential for specific import pathways in living human cells is unclear. We used RNA interference technology to specifically down-regulate the expression of(More)
Assessing the quantity of U(VI) that participates in sorption/desorption processes in a contaminated aquifer is an important task when investigating U migration behavior. U-contaminated aquifer sediments were obtained from 16 different locations at a former U mill tailings site at Naturita, CO (U.S.A.) and were extracted with an artificial groundwater, a(More)
HIV-1 employs the cellular nuclear import machinery to actively transport its preintegration complex (PIC) into the nucleus for integration of the viral DNA. Several viral karyophilic proteins and cellular import factors have been suggested to contribute to HIV-1 PIC nuclear import and replication. However, how HIV interacts with different cellular(More)
This paper presents the building of lightweight tensile structures with quadrocopters. The construction elements (such as ropes, cables, and wires) in this kind of structure are subject to tension forces. This paper identifies the basic building elements (nodes, links) required for the construction of tensile structures, and translates them into meaningful(More)
We investigated the formation of native complexes between simian virus 40 large T antigen and the cellular protein p53 (T-p53) by using simian virus 40 tsA58-transformed mouse fibroblasts (tsA58 F2b). We observed that newly synthesized p53 bound to all structural subclasses of large T antigen detectable on sucrose density gradients. This led to various(More)