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H1 and HMGB1 bind to linker DNA in chromatin, in the vicinity of the nucleosome dyad. They appear to have opposing effects on the nucleosome, H1 stabilising it by "sealing" two turns of DNA around the octamer, and HMGB1 destabilising it, probably by bending the adjacent DNA. Their presence in chromatin might be mutually exclusive. Displacement/replacement(More)
Phosphorylated rabbit cardiac alpha alpha-tropomyosin has been prepared either enzymatically (Montgomery, K., and Mak, A.S. (1984) J. Biol. Chem. 259, 5555-5560) or by fractionation of the phosphorylated and nonphosphorylated forms on a Mono Q column in 9 M urea, 50 mM Tris, pH 8.0. Although the phosphorylated and nonphosphorylated forms showed no(More)
Smooth muscle caldesmon was phosphorylated in vitro by sea star p44mpk up to 2.0 mol of phosphate/mol of protein at both Ser and Thr residues. The phosphorylation sites were contained mainly in the COOH-terminal 10-kDa cyanogen bromide fragment which houses the binding sites for calmodulin, tropomyosin, and F-actin. Tryptic peptide maps of 32P-labeled(More)
The broad family of LEA proteins are intrinsically disordered proteins (IDPs) with several potential roles in desiccation tolerance, or anhydrobiosis, one of which is to limit desiccation-induced aggregation of cellular proteins. We show here that this activity, termed molecular shield function, is distinct from that of a classical molecular chaperone, such(More)
We present a novel Markov chain Monte Carlo (MCMC) algorithm that generates samples from transdimensional distributions encoding complex constraints. We use factor graphs, a type of graphical model, to encode constraints as factors. Our proposed MCMC method, called locally annealed reversible jump MCMC, exploits knowledge of how dimension changes affect the(More)
In this paper we present a corpus of interactions of older and younger users with nine different dialogue systems. The corpus has been fully transcribed and annotated with dialogue acts and " Information State Update " (ISU) representations of dialogue context. Users not only underwent a comprehensive battery of cognitive assessments, but they also rated(More)
[1] In this study, ultraviolet TOMS (Total Ozone Mapping Spectrometer) satellite data for SO 2 are re-evaluated for the first 15 days following the 15 June 1991 Pinatubo eruption to reflect new data retrieval and reduction methods. Infrared satellite SO 2 data from the TOVS/HIRS/2 (TIROS (Television Infrared Observation Satellite) Optical Vertical(More)
The Drosophila and plant (maize) functional counterparts of the abundant vertebrate chromosomal protein HMGB1 (HMG-D and ZmHMGB1, respectively) differ from HMGB1 in having a single HMG box, as well as basic and acidic flanking regions that vary greatly in length and charge. We show that despite these variations, HMG-D and ZmHMGB1 exist in dynamic assemblies(More)
This paper proposes nonparametric regression tests of constraints involving first and second derivatives of any model E(ylX)=F(X), where the true function F is ulown. he tests are based on a statistical characterization of the departures from the constraint. The test statistics are averages computed using data 'n y and X and knowledge of the marginal(More)
Atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP), two distinct members of the natriuretic peptide family, share many features in common. However, differences in expression indicate that the processing mechanisms must be different. The leader sequence of rat BNP contains three potential phosphorylation sites for proline-directed kinases(More)