Matthew Watson

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[1] In this study, ultraviolet TOMS (Total Ozone Mapping Spectrometer) satellite data for SO2 are reevaluated for the first 15 days following the 15 June 1991 Pinatubo eruption to reflect new data retrieval and reduction methods. Infrared satellite SO2 data from the TOVS/HIRS/2 (TIROS (Television Infrared Observation Satellite) Optical Vertical Sounder/High(More)
H1 and HMGB1 bind to linker DNA in chromatin, in the vicinity of the nucleosome dyad. They appear to have opposing effects on the nucleosome, H1 stabilising it by "sealing" two turns of DNA around the octamer, and HMGB1 destabilising it, probably by bending the adjacent DNA. Their presence in chromatin might be mutually exclusive. Displacement/replacement(More)
Phosphorylated rabbit cardiac alpha alpha-tropomyosin has been prepared either enzymatically (Montgomery, K., and Mak, A.S. (1984) J. Biol. Chem. 259, 5555-5560) or by fractionation of the phosphorylated and nonphosphorylated forms on a Mono Q column in 9 M urea, 50 mM Tris, pH 8.0. Although the phosphorylated and nonphosphorylated forms showed no(More)
Several hormones that regulate nutritional status also impact on bone metabolism. Preptin is a recently isolated 34-amino acid peptide hormone that is cosecreted with insulin and amylin from the pancreatic beta-cells. Preptin corresponds to Asp(69)-Leu(102) of pro-IGF-II. Increased circulating levels of a pro-IGF-II peptide complexed with IGF-binding(More)
Smooth muscle caldesmon was phosphorylated in vitro by sea star p44mpk up to 2.0 mol of phosphate/mol of protein at both Ser and Thr residues. The phosphorylation sites were contained mainly in the COOH-terminal 10-kDa cyanogen bromide fragment which houses the binding sites for calmodulin, tropomyosin, and F-actin. Tryptic peptide maps of 32P-labeled(More)
The mechanism underlying negative regulation of HMGB1-DNA interaction by the acidic C-terminal tail is ill defined. To address this issue, we have devised a novel NMR chemical-shift perturbation mapping strategy to elucidate interactions between the tail, which consists solely of aspartic acid and glutamic acid residues, and the two well characterized(More)
We present a novel Markov chain Monte Carlo (MCMC) algorithm that generates samples from transdimensional distributions encoding complex constraints. We use factor graphs, a type of graphical model, to encode constraints as factors. Our proposed MCMC method, called locally annealed reversible jump MCMC, exploits knowledge of how dimension changes affect the(More)
We evaluated two strategies for alleviating working memory load for users of voice interfaces: presenting fewer options per turn and providing confirmations. Forty-eight users booked appointments using nine different dialogue systems, which varied in the number of options presented and the confirmation strategy used. Participants also performed four(More)
[1] Pinatubo’s 15 June 1991 eruption was Earth’s largest of the last 25 years, and it formed a substantial volcanic cloud. We present results of analysis of satellite-based infrared remote sensing using Advanced Very High Resolution Radiometer (AVHRR) and TIROS Operational Vertical Sounder/High Resolution Infrared Radiation Sounder/2 (TOVS/HIRS/2) sensors,(More)