Matthew Sprague

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For humans a daily intake of up to 500 mg omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA) is recommended, amounting to an annual requirement of 1.25 million metric tonnes (mt) for a population of 7 billion people. The annual global supply of n-3 LC-PUFA cannot meet this level of requirement and so there is a large gap between supply and(More)
a Institute of Aquaculture, School of Natural Sciences, University of Stirling, Stirling FK9 4LA, United Kingdom b Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden AL5 2JQ, United Kingdom c Biomar Ltd., North Shore Road, Grangemouth FK3 8UL, United Kingdom d Aquaculture Research Group, University of Las Palmas de Gran(More)
The replacement of fish oil (FO) with a DHA-rich Schizochytrium sp. algal meal (AM) at two inclusion levels (11% and 5.5% of diet) was tested in Atlantic salmon post-smolts compared to fish fed a FO diet of northern (NFO) or southern hemisphere (SFO) origin. Fish were preconditioned prior to the 19-week experimental feeding period to reduce long-chain(More)
In vertebrates, selenium (Se) is an essential micronutrient for vertebrates that is involved in antioxidant protection and thyroid hormone regulation among other roles and functions through its incorporation into proteins, the selenoproteins. Long-chain polyunsaturated fatty acids, such as docosahexaenoic acid (DHA), are essential nutrients for fish(More)
As the global population and its demand for seafood increases more of our fish will come from aquaculture. Farmed Atlantic salmon are a global commodity and, as an oily fish, contain a rich source of the health promoting long-chain omega-3 fatty acids, eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids. Replacing the traditional finite marine(More)
Vegetable oils (VO) are possible substitutes for fish oil in aquafeeds but their use is limited by their lack of omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA). However, oilseed crops can be modified to produce n-3 LC-PUFA such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, representing a potential option to fill the gap between(More)
Lipid class, fatty acid and POP levels were measured in migrating Atlantic bluefin tuna (ABT) tissues caught off the Barbate coast, Spain. Tissue lipids were largely characterized by triacylglycerol, reflecting large energy reserves accumulated prior to reproductive migration. Fatty acid compositions of muscle, liver and adipose exhibited similar profiles,(More)
Etched frozen hydrated specimens of mouse small intestine have been examined with low temperature scanning electron microscopy as a preliminary to X-ray microanalysis. Recognizable images have been obtained of most of the known histological features of the gut. Nuclear and cytoplasmic details were often seen. Ice crystal damage was evident, although the(More)
Omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA) are essential components of the diet of all vertebrates. The major dietary source of n-3 LC-PUFA for humans has been fish and seafood but, paradoxically, farmed fish are also reliant on marine fisheries for fish meal and fish oil (FO), traditionally major ingredients of aquafeeds. Currently, the(More)
Assessment of national dietary guidelines in a number of European countries reveals that some are based on cohort studies, focusing on total seafood consumption, while others are based on the content of EPA and DHA, distinguishing between oily and other fish. The mean actual intake of fish in most countries is around or below the recommended intake, with(More)