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Proteoglycans (PGs) in bovine corneal stroma were stained with Cupromeronic Blue in 'critical-electrolyte-concentration' (CEC) methods for electron microscopy, and were located vis-à-vis collagen fibril a-e banding patterns. Keratanase and chondroitin ABC lyase digestion showed that a + c-band- and d + e-band-associated PGs were keratan sulphate-rich and(More)
The application to connective tissues etc. of new histochemical and ultrastructural methods involving consecutive treatment with dye-salt baths, specific enzymes, antibodies and decalcifying fluids, is best carried out on sections on glass slides. This ensures uniform access of reagents to substrates and allows the accurate monitoring of the results, as(More)
Proteoglycans (PGs), stained for electron microscopy with Cupromeronic blue, were observed in skin and tendon from normal and dermatosparactic calves. Very frequently they (i.e. dermatan sulphate (DS) PGs) were seen arrayed orthogonally to the collagen fibrils, in the gap zone, usually at the d band, in both diseased and normal tissues. Where UO2(2+)(More)
Slippery slope arguments (SSAs) of the form if A, then C describe an initial proposal (A) and a predicted, undesirable consequence of this proposal (C) (e.g., "If cannabis is ever legalized, then eventually cocaine will be legalized, too"). Despite SSAs being a common rhetorical device, there has been surprisingly little empirical research into their(More)
BACKGROUND The integrative cognitive model of mood swings proposes that mood symptoms are driven by extreme, self-referent appraisals. For example, if activated mood is appraised positively, this prompts selection of mood regulation strategies that act to up-regulate mood. Appraisals are driven by fast and automatic Type 1 cognitive processes, which, left(More)
The association of proteoglycans with type I collagen fibrils in skin, tendon, cornea and bone has been determined by electron microscopy using an electron-dense dye, Cupromeronic blue, in the critical electrolyte concentration mode, backed up by biochemical analysis and digestion by hyaluronidase or keratanase. A major proteoglycan of the soft tissues,(More)
Proteoglycans (PGs) in rabbit corneal stroma and mouse sclera have been stained for electron microscopy with Cupromeronic blue in a critical electrolyte concentration (CEC) mode, with and without prior digestion of the tissue by keratanase or chondroitinase ABC to remove the keratan sulphate (KS) or chondroitin-dermatan sulphates (CS or DS) respectively.(More)
Corneas from mouse, rat and rabbit were analysed quantitatively and/or qualitatively for collagen and acid glycosaminoglycans. They were examined by light and electron microscopy, using Alcian blue and Cupromeronic blue, in critical electrolyte concentration methods, with or without digestion by hyaluronidase, chondroitinases and keratanase, for their(More)
BACKGROUND Evidence to guide clinical management of self-harm is sparse, trials have recruited selected samples, and psychological treatments that are suggested in guidelines may not be available in routine practice. AIMS To examine how the management that patients receive in hospital relates to subsequent outcome. METHODS We identified episodes of(More)
Autofluorescent granules are present in the villous syncytiotrophoblast of the human placenta, most prominently during the first trimester. These granules differ in both size and hue from classical lipofuscin granules, have different staining characteristics, and are shown by electron microscopy to be syncytial lipid droplets. It is concluded that previous(More)