• Publications
  • Influence
Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components
TLDR
It is demonstrated that copper is needed for GH61 maximal activity and that the formation of cellodextrin and oxidized cellodesxtrin products by GH61 is enhanced in the presence of small molecule redox-active cofactors such as ascorbate and gallate. Expand
Oxidoreductive Cellulose Depolymerization by the Enzymes Cellobiose Dehydrogenase and Glycoside Hydrolase 61
TLDR
It is demonstrated that the binary combination of Thermoascus aurantiacus GH61A (TaGH61A) and Humicola insolens CDH (HiCDH) cleaves cellulose into soluble, oxidized oligosaccharides, which may constitute an important, but overlooked, biological oxidoreductive system that functions in microbial lignocellulose degradation and has applications in industrial biomass utilization. Expand
Biomass Converting Enzymes as Industrial Biocatalysts for Fuels and Chemicals: Recent Developments
The economic utilization of abundant lignocellulosic biomass as a feedstock for the production of fuel and chemicals would represent a profound shift in industrial carbon utilization, allowingExpand
Heterodimerization of CCR2 Chemokines and Regulation by Glycosaminoglycan Binding*
TLDR
Results indicate that some CCR2 ligands can form stable heterodIMers in preference to homodimers and that these interactions, like those of homo-oligomers, can be influenced by some GAGs. Expand
Chemokine-Glycosaminoglycan Binding
TLDR
A mass spectrometry assay that was developed to detect protein-oligosaccharide noncovalent complexes, in this case chemokine-GAG interactions, and to select for high affinity GAGs indicates that these chemokines could be differentiated into two groups based on the stoichiometry of their complexes with the heparin oligosACcharides. Expand
Origin of Initial Burst in Activity for Trichoderma reesei endo-Glucanases Hydrolyzing Insoluble Cellulose*
TLDR
It is proposed that the frequency of structures on the substrate surface that cause transient in activation determine the extent of the burst phase of endo-glucanases, and it is suggested that the slowdown is linked to transient inactivation of enzyme on the cellulose surface. Expand
Differentiation of 3-O-sulfated heparin disaccharide isomers: Identification of structural aspects of the heparin CCL2 binding motif
TLDR
Using tandem mass spectrometry, it is shown that an octasaccharide with 11 sulfate groups with high affinity for inflammatory chemokine CCL2 does not contain 3-O-sulfated disaccharides. Expand
Cloning, expression, and characterization of a cellobiose dehydrogenase from Thielavia terrestris induced under cellulose growth conditions.
TLDR
It is shown that a CDH is present in the secretome of the thermophilic ascomycete Thielavia terrestris when grown with cellulose, along with a mixture of cellulases and hemicellulases capable of breaking down lignocellulosic biomass. Expand
CCR2 Chemokines Bind Selectively to Acetylated Heparan Sulfate Octasaccharides*
TLDR
The results indicate that HS octasaccharide sulfation is the foremost requirement for chemokine binding, however, within octAsaccharides of constant charge density, acetylation is also observed to augment binding, suggesting that there may be as yet undiscovered specificity in the chemokines-HS interaction. Expand
...
1
2
3
...