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Antibodies against purified NADP-isocitrate dehydrogenase from pig liver cytosol and pig heart were raised in rabbits. The purified enzymes from these sources are different proteins, as demonstrated by differences in electrophoretic mobility and absence of crossreactivity by immunotitration and immunodiffusion. The NADP-isocitrate dehydrogenase in the(More)
Cytoglobin (Cygb) was investigated for its capacity to function as a NO dioxygenase (NOD) in vitro and in hepatocytes. Ascorbate and cytochrome b(5) were found to support a high NOD activity. Cygb-NOD activity shows respective K(m) values for ascorbate, cytochrome b(5), NO, and O(2) of 0.25 mm, 0.3 microm, 40 nm, and approximately 20 microm and achieves a(More)
The uptake of a substrate that is transported into the cell by facilitated diffusion and subsequently converted in a series of enzymatic reactions, measured as a function of the external concentration, does not usually show the rectangular hyperbolic function characteristic of Michaelis-Menten kinetics. Instead, a seemingly biphasic curve is observed,(More)
Estuarine crustaceans are often exposed to low dissolved O2 (hypoxia) accompanied by elevated CO2 (hypercapnia), which lowers water pH. Acclimatory responses to hypoxia have been widely characterized; responses to hypercapnia in combination with hypoxia (hypercapnic hypoxia) are less well known. Here we used oligonucleotide microarrays to characterize(More)
Splice variant and authentic mRNAs for procathepsin E were measured at a ratio of 5:1 and 1:2 in Kato 3 and AGS cells, two human gastric adenocarcinoma cell lines. As a result of the precise splicing of the 3'-end of exon 6 to the 5'-end of exon 8, the variant lacked the 142 bp of exon 7 which encodes the second of the Asp residues that operate the(More)
The pmclient and pmserver programs are used to measure the network traffic conditions between two given nodes on the Planet-Lab network. By running the pmclient program on a Planet-Lab node, and the pmserver program on a corresponding node, the two programs produce a dynamic flow of traffic in the form of UDP packets and optionally IPv4 ICMP echo packets(More)
The two human pronapsin genes which are located immediately adjacent to one another on chromosome 19 were shown, using quantitative RT-PCR, to have distinctly different expression patterns. Transcription of the pronapsin A gene in normal human lung tissue was not appreciably altered in lung carcinomas and comparable pronapsin A mRNA levels were also(More)
Past attempts to get computers to ride bicycles have required an inordinate amount of learning time (1700 practice rides for a reinforcement learning approach [1], while still failing to be able to ride in a straight line), or have required an algebraic analysis of the exact equations of motion for the specific bicycle to be controlled [2, 3]. Mysteriously,(More)
Optical see-through head-mounted displays are currently seeing a transition out of research labs towards the consumer-oriented market. However, whilst availability has improved and prices have decreased, the technology has not matured much. Most commercially available optical see-through head mounted displays follow a similar principle and use an optical(More)