Matthew B. Hancock

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Serotonin-immunoreactive fibers have been observed in the dorsal columns of the golden hamster spinal cord. The fibers were visualized using the PAP method which was intensified with a DAB-nickel substrate. The fibers were arranged preferentially in the mid-sagittal plane, and many were observed coursing from the dorsal columns into the dorsal gray matter.(More)
The retrograde transport of horseradish peroxidase (HRP) was used to determine the distribution of hypothalamic neurons which send axonal projections to the spinal cord. Labeled cells were observed in the paraventricular nucleus (PV) and in the lateral hypothalamic area following HRP injections into the lower thoracic and upper lumbar spinal cords of rats.(More)
A method for differentially staining for two antigens in single sections is described. Paraffin-embedded or Vibratome sections are incubated in two sequences of immunoperoxidase (PAP) reagents using a diaminobenzidine (DAB)-nickel ammonium sulfate solution to localize the first antigen, and DAB alone to localize the second antigen. With these chromagens,(More)
Calcitonin gene-related peptide (CGRP)- and somatostatin (SRIF)-containing cells were identified by immunocytochemical techniques in pancreatic islet cells of the rat. CGRP-containing cells were found primarily in the peripheral portion of the pancreatic islets. In addition, CGRP-containing cells also contained somatostatin, which identifies the islet(More)
  • M B Hancock
  • The journal of histochemistry and cytochemistry…
  • 1984
The sequential application of the peroxidase-antiperoxidase (PAP) technique with nickel-intensified DAB and with DAB alone was used to visualize black peptide-immunoreactive endings on amber serotonin-immunoreactive cells in 1-2-micron paraffin sections of the hamster medulla. Met-enkephalin- and substance P-positive terminals were present on serotonin(More)
The new avidin-biotin-peroxidase complex (ABC) technique was used to localize the [D-Lys6] analog of gonadotropin releasing hormone (GnRH), labeled with biotin, on pituitary monolayer cultures from female rats. Staining was diffuse, or in patches, on the surface of 10-17% of the cells 30 sec-3 min after the addition of 10(-10)-10(-12) M biotin-labeled GnRH.(More)
These studies describe the application of new cytochemical stains that co-localize a biotin-labeled gonadotropin releasing hormone (GnRH) analog and FSH or LH in the same field or cell. Pituitary monolayer cells were stimulated with the [D-Lys6] GnRH analog or the same analog labeled with biotin. Biotinylated [D-Lys6] GnRH exhibited a higher affinity and(More)
Following the iontophoretic deposition of Phaseolus vulgaris leucoagglutinin (PHA-L) into the rostral medullary raphe, which included portions of the caudal nucleus raphe magnus, rostral nucleus raphe pallidus, rostral nucleus raphe obscurus and rostral nucleus reticularis paragigantocellularis, two-color immunoperoxidase staining was employed to(More)
The distribution in the spinal cord of the rat of preganglionic neurons sending fibers into the hypogastric nerve was determined with the retrograde transport of horseradish peroxidase (HRP). Labeled cells were present in the intermediate gray matter of spinal segments L1-L2. The majority (81%) of HRP-filled cells formed a continuous column along the(More)