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The protease-activated family of G protein-coupled receptors includes PAR-1 and PAR-3, which are activated by thrombin, and PAR-2, which is activated by trypsin and tryptase. PAR-2 has recently been shown to be expressed in human endothelial cells. In the present studies, we have examined the expression of PAR-2 in other cells, particularly vascular smooth(More)
Biological analysis in an on-chip flow cytometry is predictable, which often works with small volumes of reagents. A microfluidic chip to mix and encapsulate both living cells and drug in picoliter aqueous drops is proposed. By the aid of a counter-flow unit and a T-junction, this chip can mix cells with drugs, and form them into droplets of liquid in the(More)
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