Matt Klos

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RATIONALE Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) offer a powerful in vitro tool to investigate disease mechanisms and to perform patient-specific drug screening. To date, electrophysiological analysis of iPSC-CMs has been limited to single-cell recordings or low-resolution microelectrode array mapping of small cardiomyocyte(More)
The effect of Entamoeba histolytica extracts (E.h.e.) on proliferation in vitro of human peripheral blood lymphocytes, cord blood lymphocytes, and lymphocytes enriched for T cells or non-T cells was investigated. As tested by 3H-thymidine uptake and by morphologic criteria, E.h.e. stimulate T lymphocyte proliferation. Non-T lymphocytes do not respond to(More)
A 6-yr-old girl with congenital corticosteroid-resistant pure red cell aplasia was treated with bone marrow transplant from her HLA-identical, MLC-unreactive sister in November 1984 following conditioning with busulfan and cyclophosphamide. Full engraftment was obtained and the patient at 21 months post-transplant is in excellent clinical condition(More)
Suppressor T cells of humoral immune responses, effector T cells mediating DTH, suppressor T cells of DTH, and helper T cells of humoral immune responses, all with specificity to SRBC, were produced in mice. The biologic activity was tested in adoptive transfer experiments. In vitro treatment with different doses of 4-hydroperoxycyclophosphamide (4-HPCy)(More)
BACKGROUND Persistent atrial fibrillation (PAF) results in electromechanical and structural remodeling by mechanisms that are poorly understood. Myofibroblast proliferation and fibrosis are major sources of structural remodeling in PAF. Myofibroblasts also interact with atrial myocytes via direct physical contact and release of signaling molecules, which(More)
BACKGROUND Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) monolayers generated to date display an immature embryonic-like functional and structural phenotype that limits their utility for research and cardiac regeneration. In particular, the electrophysiological function of hPSC-CM monolayers and bioengineered constructs used to date are(More)
Chitin, a linear beta 1-4 linked poly-N-acetylglucosamine, enhanced the in vivo immune response of normal mice to sheep red blood cells, but not that of mice with thymic aplasia (nude mice). The compound is mitogenic for murine B lymphocytes but not for thymocytes or splenic T lymphocytes. Similarly to well-known polyclonal B-lymphocyte activators; e.g.(More)
The effect of phorbol myristate acetate (PMA) on lectin-induced murine thymocyte activation was studied. PMA itself failed to stimulate thymocyte proliferation, but potentiated concanavalin A (Con A)-induced thymocyte activation. A brief incubation of thymocytes with PMA changed the responsiveness of these cells to an optimal mitogenic dose of Con A present(More)