Learn More
Ex vivo-expanded mesenchymal stem cells (MSCs) were transduced with a green fluorescent protein (GFP) retroviral construct and subsequently infused into 3 adult baboons following lethal total body irradiation and hematopoietic support or without any prior conditioning. To study the long-term fate of these MSCs, necropsies were performed between 9 and 21(More)
Bluetongue virus multiplied in cell lines derived fromAedes albopictus andAedes pseudoscutellaris cells. Virus reached a maximum titre in theAe. pseudoscutellaris cells three days post inoculation, and inAe. albopictus cells six days p. i. Virus growth was demonstrated in both cell lines at 27° C and 37° C. Significant titres of virus were still present in(More)
Type 9 African horse-sickness virus multiplied to a high titre in bothCulicoides nubeculosus andC. variipennis after intrathoracic inoculation and inC. variipennis after oral ingestion. The orally infectedC. variipennis were able to transmit the virus by biting after 13 days incubation at 26° C but not after 6 days incubation. Intrathoracically inoculatedC.(More)
Akabane virus replicated in Culicoides nubeculosus and Culicoides variipennis after intrathoracic inoculation and was maintained in both species of midge for at least 9 days post-infection. The virus also replicated to high concentration in C. variipennis after oral infection and was transmitted through a membrane by this species of midge 7-10 days after(More)
Membrane feeding and intrathoracic inoculation techniques were used to infect Phlebotomus papatasi sandflies with Phlebotomus fever group viruses. Sandflies were orally infected with Pacui virus, and were inoculated with Pacui, Sicilian sandfly fever and Naples sandfly fever viruses. Pacui virus did not multiply in these insects after oral infection, but an(More)
Seven-day-old embryonated hen eggs were infected with African Horse Sickness virus by the yolk sac and intravenous routes. Virus reached a high titre in the blood of infected embryos.Culicoides variipennis midges which took a blood meal from infected eggs became infected with virus, and after 7 days at 26°–27° C transmitted African Horse Sickness virus to(More)
The use ofToxorhynchites brevipalpis as a system for the propagation and isolation of bluetongue virus (BTV) was investigated. BTV was found to multiply inT. brevipalpis after infection by intrathoracic inoculation. Virus concentrations of up to 6.9 log10 TCID50 per mosquito were found within 7 days of infection and were maintained for at least 6 days.(More)
Oral infection of haematophagous insects with arboviruses to test their vector competence can be a difficult procedure in the laboratory since "wild caught" insects often prove reluctant to feed, once captured. This paper describes a technique using virus-charged glass needles to infect Culicoides spp. orally with bluetongue virus (BTV). C. variipennis, a(More)