Mathieu Bach

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Antinuclear autoantibodies are a hallmark of autoimmune diseases. The RNP, Sm and SS-B nuclear antigens from calf thymus in whole tissue, nuclear extracts and fractions have been studied by using different techniques including immunodiffusion, counterimmunoelectrophoresis and protein blotting. Such studies were done in order to obtain a precise(More)
PepX is a X-prolyl dipeptidyl aminopeptidase of S15 family that cleaves dipeptides from the N-terminus of polypeptides having a proline or alanine residue at the second position. Involved in bacterial nutrition and in peptide maturation, this serine exopeptidase, counterpart of the mammalian DDP-4, has been proposed to play a role in pathogenicity for(More)
A multiple sample immunoblotting system (MSIS) is described. The MSIS permits the detection and classification of antinuclear antibodies (ANA) according to the pattern of antigenic polypeptides recognized in four different extracts: calf thymus whole tissue, nuclear extract, and two ammonium sulphate fractions of the nuclear extract. The procedure permits(More)
A procedure is described allowing the easy and fast obtention of a cellular extract from calf thymus, enriched in the undegraded 52 kDa SS-B protein. As seen by western blot, the extract does not contain Sm and RNP antigens, allowing the use of such fraction for the detection of anti-SS-B antibodies without the interference of the anti-Sm and anti-RNP(More)
Stable degradation products, obtained by digestion with endogenous and V8 proteases of calf thymus SS-B (La) antigenic protein, have been studied. The most characteristic fragments have molecular masses of 47, 30, 23 and 17 kDa. The 47-kDa and 30-kDa fragments are complex and are constituted of a number of species of different isoelectric points, as has(More)
RNP, Sm and SS-B nuclear antigens from calf thymus were studied with respect to the size distribution on sucrose gradients as well as to the molecular integrity and related structural changes when they were subjected to enzymatic digestions under different conditions. Making a difference with RNP particles, the Sm size distribution is concentration(More)
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