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ABSTRACT Competition among eight Rhynchosporium secalis isolates was assessed during parasitic and saprophytic phases of the disease cycle in field experiments conducted at two locations and over two growing seasons. The eight isolates were inoculated onto six barley populations exhibiting varying degrees of resistance. Microsatellite analysis of 2,866… (More)
Highly virulent, slow-growing grey (SGG); moderately virulent, fast-growing salmon (FGS); and avirulent/weakly virulent, fast-growing grey (FGG) forms of Colletotrichum gloeosporioides have been described from yam (Dioscorea spp.), but little is known about their chemodiversity or the role of toxins in their pathogenesis. Secondary metabolite profiles in… (More)
2009. QTL analysis for acochyta blight rsistance in cickpea (Cicer arietinum L.) uing mcrosatellite mrkers. Microsatellites have become markers of choice for molecular mapping and marker assisted selection of key traits such as disease resistance in many crop species. The aim of this study was to construct a genetic linkage map and analyze quantitative… (More)
Dioscorea (true yams) is a large genus that contains species important as food (with edible tubers) or as sources of bioactive substances used in a range of applications. Dioscorea is a major staple food in many parts of the world, especially in West Africa and the Pacific islands, and serves as a famine food in many regions. It is a critically important… (More)
Genetic variability among 122 Rhynchosporium secalis isolates collected from barley in three regions of Tunisia was investigated using host differentials, amplified fragment length polymorphism (AFLP), and microsatellite markers. The isolates were collected from a widely grown scald-susceptible barley cultivar Rihane and a range of local landrace cultivars… (More)
Recent developments in agricultural science and technology have the potential to transform the agricultural sector in the developing world. These technological advances constitute key drivers of economic growth and hold great promise for poverty reduction in sub-Saharan Africa (SSA). Agricultural research and development in Africa is undergoing a major… (More)
This protocol describes how to produce yam (Dioscorea) seeds through controlled pollination of flowers on female plants. Pollination by flower thrips takes place naturally in normal yam-growing environments in the presence of flowering male and female genotypes, although it is not efficient. This protocol leads to the production of hybrid seeds of known… (More)
This protocol describes how to extract DNA from the leaves of true yams (Dioscorea) for polymerase chain reaction (PCR) and other analyses.
This protocol describes how to produce whole yam (Dioscorea) plantlets in vitro through yam meristem culture and from yam node cuttings.