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PURPOSE The experiment was performed to prove that exposure to a relatively weak extremely low frequency (ELF) magnetic field retards tadpoles' development. METHODS Two cohorts of Xenopus laevis laevis (Daudin) tadpoles were exposed during their immature period ( approximately 60 days) to a 50 Hz magnetic field of 63.9 < or = B < or = 76.4 microT rms(More)
To determine the activity of matrix metalloproteinases (MMP), especially MMP-2 and MMP-9, which play an important role in ischemic stroke and intracerebral hemorrhage, we adapted a simple and rapid method for localizing gelatinase activity to a gelatin film in situ-overlay technique previously used in cancer research. Ten micrometer cryosections of rat(More)
Overview: In driving hES cell technology towards widespread applications considerable effort has been focused on the improvement of culture conditions and on enabling efficient differentiation. We have established two technologies which will better enable researchers to achieve these aims. The use of feeder based protocols for the creation, expansion and(More)
Heart and vascular endothelial cells from human pluripotent stem cells are of interest for applications in cell therapy and cardiovascular disease. Differentiation protocols are now sufficiently refined that production of cardiomyocytes, endothelial-and smooth muscle cells is fairly efficient and reproducible. Genetically marked hESCs have been produced in(More)
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