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Characterization of NADH oxidase/NADPH polysulfide oxidoreductase and its unexpected participation in oxygen sensitivity in an anaerobic hyperthermophilic archaeon.
Many genomes of anaerobic hyperthermophiles encode multiple homologs of NAD(P)H oxidase that are thought to function in response to oxidative stress. We investigated one of the seven NAD(P)H oxidaseExpand
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Template-directed DNA photoligation via alpha-5-cyanovinyldeoxyuridine.
[reaction: see text] We describe an efficient template-directed photoligation of oligodeoxynucleotides (ODNs) using alpha-5-cyanovinyldeoxyuridine (alpha(C)U). An efficient photoligation was producedExpand
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Highly selective and sensitive template-directed photoligation of DNA via 5-carbamoylvinyl-2'-deoxycytidine.
We describe a highly efficient template-directed photoligation of oligodeoxynucleotides (ODNs) through 5-carbamoylvinyl-2'-deoxycytidine ((CV)C). When an ODN containing (CV)C at the 5' end wasExpand
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Detection of methylcytosine by DNA photoligation via hydrophobic interaction of the alkyl group
We report the nonenzymatic detection of 5-methylcytosine by using template-directed photoligation through 5-vinyl-2′-deoxyuridine derivatives with high selectivity. In this paper, we propose a newExpand
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Highly selective detection of 5-methylcytosine using photochemical ligation.
We report the nonenzymatic detection of 5-methylcytosine by using template-directed photoligation through 5-cyanovinyl-2'-deoxyuridine ((C)U) with high selectivity and present a new methylationExpand
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Replication of cyclobutane pyrimidine dimer analogue by Ex Taq DNA polymerase
Abstract We previously reported an efficient and reversible template-directed photoligation using 5-carboxyvinyl-2′-deoxyuridine (CVU)-containing ODN at the 5′-terminal. This method forms d(T-CVU) asExpand
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Photochemical Site-specific Mutation of 5-Methylcytosine to Thymine
In this study, we report that the coupling of 5-methylcytosine with 5-carbamoylvinyl-deoxyuridine-mediated photoligation caused the heat-induced transition from 5-methylcytosine to thymine. Deamina...
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Effective synthesis of photosensitive oligodeoxynucleotides.
In this paper, the synthesis of various vinyl substituted photosensitive pyrimidine nucleosides and nucleotides is described; starting from 5-Iodo-2'-deoxyuridine (IdU) or oligodeoxynucleotides (ODN)Expand
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A photochemical detection of methylcytosine by using hydrophobic interaction.
In this paper, the nonenzymatic detection of 5-methylcytosine with high selectivity is described. We present a new methylation detection method by using template-directed photoligation throughExpand
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