Masatoshi Aoki

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The seedlings of Pinus armandi Franch. were exposed to ozone (O(3)) at 300 ppb for 8 h a day, 6 days a week, and simulated acid rain of pH 3.0 or 2.3, 6 times a week, alone or in combination, for 14 weeks from 15 June to 20 September 1993. The control seedlings were exposed to charcoal-filtered air and simulated rain of pH 6.8 during the same period.(More)
Flavobacterium psychrophilum infection in salmonid fish, known as rainbow trout fry syndrome (RTFS) or bacterial coldwater disease (BCWD), is widespread in fish farms and natural waters. Despite many studies in which attempts at infection were made, an adequate method of infection has not yet been established. In this study, we evaluated a bath infection(More)
Flavobacterium psychrophilum is the causative agent of bacterial cold water disease (BCWD) and rainbow trout (Oncorhynchus mykiss) fry syndrome (RTFS). Logarithmic phase formalin-killed cells (FKC) of F. psychrophilum induced immunity to BCWD in ayu (Plecoglossus altivelis) by using an oral administration. In this study, we investigated the effective(More)
Immunoglobulin of the torafugu, Takifugu rubripes, was purified by a combination of precipitation by low ionic strength dialysis and gel filtration. The Ig was used to immunise mice for the production of monoclonal antibody (MAb). Supernatants of hybridoma cultures were screened by enzyme-linked immunosorbent assay using purified-torafugu Ig-coated plates,(More)
To investigate the effects of ambient-level gas-phase peroxides concurrent with O(3) on foliar injury, photosynthesis, and biomass in herbaceous plants, we exposed Japanese radish (Raphanus sativus) to clean air, 50 ppb O(3), 100 ppb O(3), and 2-3 ppb peroxides + 50 ppb O(3) in outdoor chambers. Compared with exposure to 100 ppb O(3), exposure to 2-3 ppb(More)
Acid treatments significantly change the physical and chemical properties of red-yellow soil by lowering its pH value and leaching out aluminum(Al) ions that are harmful to the growth of plants. The structure of soil will be damaged, resulting in higher viscosity, higher water retention rate and lower air permeability of the soil. The germination rate of(More)
Chinese pine seedling, which age is 87 days, are exposed to SO2 in three treatments for 60 days from July 1, 2002 to August 29, 2002. In treatment 1 and treatment 2, the concentration of SO2 were 0.1 mg x m(-3) and 0.2 mg x m(-3), respectively. In treatment 3, the concentration of SO2 were 0.5 mg x m(-3) for 6 hours and 0.1 mg x m(-3) for 18 hours per day.(More)
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