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An Arabidopsis cell wall-associated kinase required for invertase activity and cell growth.
It is shown that a single wak2 mutation exhibits a dependence on sugars and salts for seedling growth, and WAKs may provide a molecular mechanism linking cell wall sensing to regulation of solute metabolism, which in turn is known to be involved in turgor maintenance in growing cells.
Ubiquity of a Borate-Rhamnogalacturonan II Complex in the Cell Walls of Higher Plants
In 15 species it seemed likely that RG-II might be the exclusive carrier of boron in the cell wall, and in all the species examined, cell-wall borons was judged to be associated withRG-II.
Immunocytochemistry of Rhamnogalacturonan II in Cell Walls of Higher Plants
Wall-associated kinase 1 (WAK1) is crosslinked in endomembranes, and transport to the cell surface requires correct cell-wall synthesis
- B. D. Kohorn, Masaru Kobayashi, Susan Johansen, H. Friedman, A. Fischer, N. Byers
- BiologyJournal of Cell Science
- 1 June 2006
This work shows that in leaf protoplasts a WAK1-GFP fusion protein accumulates in a cytoplasmic compartment that contains pectin, and that the assembly and crosslinking of WAKs may begin at an early stage within a cytopsized compartment rather than in the cell wall itself, and is coordinated with synthesis of surface cellulose.
Boron nutrition of cultured tobacco BY-2 cells. IV. Genes induced under low boron supply.
Genes whose expression was up-regulated in low boron (B)-acclimated tobacco BY-2 (Nicotiana tabacum L. cv. Bright Yellow 2) cells, which had been selected under a low supply of B, were screened by the cDNA differential subtraction method, indicating that B deficiency might impose cellular redox imbalance on the cells.
Boron Nutrition of Tobacco BY-2 Cells. V. Oxidative Damage is the Major Cause of Cell Death Induced by Boron Deprivation
The results suggest that the death of tobacco cells induced by B deprivation is not likely to be a typical PCD, suggesting that the oxidative damage is the immediate and major cause of cell death under B deficiency.
Boron deprivation immediately causes cell death in growing roots of Arabidopsis thaliana (L.) Heynh.
These results demonstrated that A. thaliana immediately senses and responds to the removal of B from the external medium, and similar responses were induced by calcium deprivation but not magnesium or potassium deprivation, suggesting that the failure of cross-linking in pectin molecules in growing cells triggers these rapid responses.
Boron Nutrition of Cultured Tobacco BY-2 Cells. II. Characterization of the Boron-Polysaccharide Complex
Results suggests that B may fulfill its essential function as forming the B-RG-II complex in cell walls in cultured tobacco BY-2 cells.
Characterization of Arabidopsis CTP:3-deoxy-D-manno-2-octulosonate cytidylyltransferase (CMP-KDO synthetase), the enzyme that activates KDO during rhamnogalacturonan II biosynthesis.
It is suggested that KDO is an indispensable component of RG-II, and that the complete B-RG-II complex is essential for the cell wall integrity of rapidly growing tissues.