Masahiro M H Osakabe

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A polymerase chain reaction (PCR)-restriction fragment length polymorphism (PCR-restriction fragment-length polymorphism)-based method for species identification was applied to 14 Tetranychus spider mite species, which were dominant species intercepted at Japanese import plant quarantine. We sequenced the internal transcribed spacer (ITS) region of the(More)
Species identification is a basic issue in biosecurity. Polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) is a useful molecular diagnostic tool for species identification. However, the lack of transferability of data has been a serious shortcoming of this method. A RFLP catalog, i.e., a graph of PCR-RFLP patterns(More)
Plant-dwelling mites are potentially exposed to solar ultraviolet-B (UVB) radiation that causes deleterious and often lethal effects, leading most mites to inhabit the lower (underside) leaf surfaces. However, in species of spider mite belonging to the Genus Panonychus, a substantial portion of individuals occur on upper leaf surfaces. We investigated(More)
Host race formation generates diversity within species and may even lead to speciation. This phenomenon could be particularly prevalent in the Acari due to the often intimate interaction these species have with their hosts. In this review, we explore the process of host race formation, whether it is likely to occur in this group and what features may favour(More)
The spatial distribution of the two-spotted spider mite Tetranychus urticae Koch is biased toward the lower surfaces of leaves as compared with the upper leaf surfaces on their host plants. Because of the deleterious effects of solar ultraviolet (UV) irradiation, we hypothesized T. urticae remains on lower leaf surfaces as an adaptation to avoid solar UV(More)
The herbivorous spider mite Tetranychus urticae usually remains on the lower leaf surfaces of its host plants. Although terrestrial animals are generally thought to be well protected from damage because of UV radiation, insect herbivory frequently increases when solar UV-B (280-315 nm) radiation is attenuated. As UV transmission through leaves is generally(More)
To determine whether the Bunsen-Roscoe reciprocity law (i.e., the extent of photochemical effects is determined by cumulative irradiance) is applicable to ultraviolet-B (UVB) damage in the twospotted spider mite Tetranychus urticae, egg hatchability and survival of individuals were assessed after irradiation with a UVB lamp using various combinations of(More)
Ambient ultraviolet-B (UVB) radiation impacts plant-dwelling arthropods including herbivorous and predatory mites. However, the effects of UVB on prey–predator systems, such as that between the herbivorous spider mite and predatory phytoseiid mite, are poorly understood. A comparative study was conducted to determine the vulnerability and behavioral(More)
Solar ultraviolet-B (UVB) radiation has deleterious effects on plant-dwelling mites. We assessed the biological effects of UVB radiation on the eggs of the twospotted spider mite, Tetranychus urticae Koch, under both near ambient (UV+) and UV-attenuated (UV−) conditions from spring to autumn and compared them to the effects of temperature and humidity. The(More)
Polyandry is more widespread than anticipated from Bateman's principle but its ultimate (evolutionary) causes and proximate (mechanistic) correlates are more difficult to pinpoint than those of polygyny. Here, we combined mating experiments, quantification of reproductive traits and microsatellite genotyping to determine the fitness implications of(More)