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RNA interference (RNAi) is a mechanism by which double-stranded RNAs (dsRNAs) suppress specific transcripts in a sequence-dependent manner. dsRNAs are processed by Dicer to 21-24-nucleotide small interfering RNAs (siRNAs) and then incorporated into the argonaute (Ago) proteins. Gene regulation by endogenous siRNAs has been observed only in organisms(More)
Imprinted genes are epigenetically marked during gametogenesis so that they are exclusively expressed from either the paternal or the maternal allele in offspring. Imprinting prevents parthenogenesis in mammals and is often disrupted in congenital malformation syndromes, tumours and cloned animals. Although de novo DNA methyltransferases of the Dnmt3 family(More)
BACKGROUND MicroRNAs (miRNAs) are critical regulators of transcriptional and post-transcriptional gene silencing, which are involved in multiple developmental processes in many organisms. Apart from miRNAs, mouse germ cells express another type of small RNA, piwi-interacting RNAs (piRNAs). Although it has been clear that piRNAs play a role in repression of(More)
BACKGROUND To investigate the functions of Dicer and microRNAs in neural stem (NS) cell self-renewal and neurogenesis, we established neural stem cell lines from the embryonic mouse Dicer-null cerebral cortex, producing neural stem cell lines that lacked all microRNAs. PRINCIPAL FINDINGS Dicer-null NS cells underwent normal self-renewal and could be(More)
This paper proposes an energy based control law for swinging up the Acrobot. The control law is designed and the convergence analysis is carried out based on Lyapunov stability theory. The conditions on the parameters in the control law such that the total energy of the Acrobot converges to the potential energy of its top upright position are given. Under(More)
BACKGROUND Argonaute2 protein (Ago2) is a key component of RNA-induced gene silencing complex, which is crucial for microRNA-mediated repression of target genes. The function of Ago2 in the mouse oocyte and early embryonic development is less well characterized but it is likely to have an important role in regulating maternally inherited mRNA. We have(More)
Imprinted genes are expressed in a parent-of-origin manner and are located in clusters throughout the genome. Aberrations in the expression of imprinted genes on human Chromosome 7 have been suggested to play a role in the etiologies of Russell-Silver Syndrome and autism. We describe the imprinting of KLF14, an intronless member of the Krüppel-like family(More)
This paper concerns a swing-up control problem for a three-link gymnastic planar robot in a vertical plane with its first joint being passive (unactuated) and the rest being active (actuated). The objectives of this paper are to: (1) design a controller under which the robot can be brought into any arbitrarily small neighborhood of the upright equilibrium(More)
— This paper studies the energy based control for swinging up two pendulums on a cart. This paper presents an original and detail analysis of the convergence of the energies of two pendulums and provides the condition such that the pendulums can be swung up. Moreover, the stability analysis of the motion of two pendulums under the energy based control is(More)
Sperm DNA damage affects the conception rate resulting from human assisted reproduction technology. The objective of this study was to adapt the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay to provide a quality parameter for bull semen based on the detection of sperm DNA damage. Fresh semen was collected from two(More)