The kinetic mechanisms of the 2-oxoglutarate and pyruvate dehydrogenease complexes from pig heart mitochondria were studied at pH 7.5 and 25 degrees. A three-site ping-pong mechanism for the actin of… (More)
Studies on the activation of an apopyruvate dehydrogenation system obtained from extracts of lipoic acid-deficient Streptococcus jaecalis cells indicated that in its functional form lipoic acid is… (More)
WHEN Pieris rapæ L. was photographed under the illumination of ultra-violet light passing through the filter UV-D1 obtained from the Matsuda Lamp Co., the wings of female butterflies, in accordance… (More)
Lipoamide dehydrogenase (reduced nicotinamide adenine dinucleotide :lipoamide oxidoreductase, EC 1.6.4.3) has been isolated from acid-precipitated human liver particles in a highly purified state by… (More)
Genomic clones encompassing the entire gene (PDH alpha) encoding the human pyruvate dehydrogenase alpha-subunit (PDH alpha) have been isolated by screening a leukocyte genomic library in the cloning… (More)
An attempt has been made to provide a description of the mammalian alpha-keto acid dehydrogenase multienzyme complexes, especially with respect to the structure, assembly and function of PDC and OGDC… (More)
Extracellular ATP, ADP and GTP increased the intracellular free Ca2+ concentration ([Ca2+]i) in a suspension of isolated rat hepatocytes. The [Ca2+]i was determined by measuring fura-2 fluorescence,… (More)
A procedure for rapid separation and microquantitative determination of various alpha-keto acids in serum and urine was developed. The procedure used reverse-phase high-performance liquid… (More)