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To target a specific region of a protein, monoclonal antibodies (mAbs) are often developed against peptide fragments representative of the target epitope on the intact protein. We report on a chemical strategy for targeting epitopes near phosphorylation sites; we developed a synthetic capture agent against the C-terminal epitope of Akt2 (or protein kinase B(More)
We describe a general synthetic strategy for developing high-affinity peptide binders against specific epitopes of challenging protein biomarkers. The epitope of interest is synthesized as a polypeptide, with a detection biotin tag and a strategically placed azide (or alkyne) presenting amino acid. This synthetic epitope (SynEp) is incubated with a library(More)
INTRODUCTION Sarcoidosis is a multi-organ system granulomatous disease of unknown origin with an incidence of 1-40/100,000. Though pulmonary manifestations are predominant, ocular sarcoidosis (OS) affects 25-50% of patients with sarcoidosis and can lead to blindness. METHODS A retrospective, single-center chart review of sarcoidosis cases investigated(More)
Conjugated fat-soluble vitamins, methylenic interrupted and conjugated fatty acids were polarographically investigated in both basic and neutral solvents. The half-wave potentials of all-trans-retinol, 13-cis-retinol, all-trans-retinyl acetate, all-trans-retinal, and Vitamin D2 and D3 were related to the number of double bonds in conjugation and their(More)
Rheumatoid arthritis (RA) is a systemic autoimmune disease that causes joint pain, inflammation, and loss of function. Disease pathogenesis involves activation and proliferation of autoreactive pro-inflammatory effector T cells. While the details of RA onset and progression remain controversial, dendritic cell (DC) numbers dramatically increase in the(More)
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