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Intravenous phenytoin has come under increased scrutiny with the introduction of the prodrug, fosphenytoin. We evaluated adverse events and length-of-stay using parenteral the two drugs in routine emergency department use. Open-label randomization of phenytoin or fosphenytoin in 256 Emergency Department patients prescribed 279 parenteral doses of a(More)
Human transferrin is shown to bind 2 mol of aluminium per mol of protein using spectrophotometric titration. Competitive equilibrium between aluminium and ferric ions for transferrin binding sites is observed, and a value of 2.5 (+/- 0.4) X 10(15) M-1 is found for the apparent binding constant under physiological conditions.
To execute a strategy for reconstituting genetic resources from cryopreserved blastodermal cells, experiments were conducted to optimize conditions for producing chimeric chickens from frozen-thawed blastodermal cells. Stage X blastodermal cells were collected from Barred Plymouth Rock embryos and dispersed. Cells were resuspended in 10% dimethyl sulfoxide(More)
Few data exist on the frequency, aetiology and outcome of cerebrovascular complications of bone marrow transplantation (BMT). We reviewed all patients undergoing BMT at the Fred Hutchinson Cancer Research Center, Seattle, Wash., USA (a large referral institution) over 3 years. We reviewed ICD-9 (International Classification of Diseases) codes for ischaemic(More)
Fundamental questions arising from study of the multiple sclerosis lesion are: (1) why the myelin-forming oligodendrocyte is adversely affected; and (2) what factors might induce the proliferation and differentiation of replacement oligodendrocytes. This paper discusses factors that influence the number and differentiative capacity of myelin-forming cells(More)
1. In subjects with normal renal function there was a strong positive correlation between serum concentrations of 25-hydroxycholecalciferol and 24,25-dihydroxycholecalciferol, as measured by competitive protein-binding assay. 2. The 24,25-dihydroxycholecalciferol concentration was about 7% of the prevailing 25-hydroxycholecalciferol concentration. 3. In(More)
To investigate the possible uptake of aluminium by plasma proteins, the Group IIIB element gallium (67Ga) was used as an analogue. Gel filtration confirmed that plasma bound 67Ga in the transferrin region, and this effect could be reproduced using pure transferrin. Approximately 75%, of 67Ga could be displaced by 10 mumol/l aluminium in the buffer.(More)
Difference UV spectrophotometry is used to determine the conditional binding constants for aluminum to human transferrin in the presence of HCO3- of initial concentration 18 mM according to a two site model. The values obtained at 37 degrees C and pH 7.4 are: K1 = 3.0 (+/- 0.8) X 10(15) M-2, K2 = 2.9 (+/- 0.7) X 10(15) M-2 for the reactions: Tr + Al3+ +(More)
Duodenums of intact anaesthetised rats have been perfused with saline-bicarbonate buffer (pH 8.5), containing AlCl3, across a range of concentrations 0-300 microM. A linear relation between rate of uptake and perfusate Al concentration was found. The clearance of Al from the lumen was also studied with the mean perfusate concentration of 60 microM, in the(More)
Metamorphosis in the flatfish is characterized by the migration of one eye around the dorsal surface of the head to a position adjacent to the other eye on the new top side of the animal. The visual connections of the adult flatfish, Achirus lineatus, were examined. Either the migrating or non-migrating eye was removed and the animal allowed to survive for(More)