Mary M. Werner

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Three new unrelated cases of PIBIDS (Photosensitivity, Ichthyosis, Brittle, sulfur-deficient hair [trichothiodystrophy], Impaired intelligence, Decreased fertility, and Short stature) are reported. Decreased survival of skin fibroblast lines after UVB exposure was found. All three male patients had hypogonadism and primary end-organ gonadal failure.(More)
Cells of wild-type Schizosacharomyces pombe exposed to UV radiation in either G1 or G2 phase show enhanced inactivation of colony-forming ability if plated in the presence of caffeine. This UV-sensitization by caffeine is abolished in both G1 and G2 phase cells by the rad1 mutation; since both caffeine and the rad1 mutation markedly reduce recombinational(More)
Inhibition of DNA repair by caffeine is manifested in Schizosaccharomyces pombe wild-type cells as an enhancement of UV- or γ-irradiation-induced lethality. The progress of DNA repair processes involving one or more caffeine-sensitive steps may be conveniently followed by measuring the concomitant decrease of this lethal enhancement effect. By measuring,(More)
The progress of repair in Schizosaccharomyces pombe may be followed during post-irradiation incubation by measuring, after various intervals, the ability of UV- or γ-irradiated cells to avoid enhanced lethality when exposed to the repair inhibitor caffeine (Gentner and Werner, 1975). This technique has now been used to investigate the effect of inhibition(More)
A synergistic effect of combined UV and γ-ray exposure was observed for inactivation of wild-type Schizosaccharomyces pombe. A recombinational repair process, known to be important in restitution of damage induced by both radiations, appears to be involved; a radiation-sensitive mutant defective in this repair pathway showed essentially no synergistic(More)
The time course of recovery in UV-or γ-irradiated wild-type Schizosaccharomyces pombe has been determined by fractionated dose experiments and by measuring the rate at which the “resistant shoulder” of the survival curve was regained during post-irradiation incubation in growth medium. The kinetics of recovery after UV-irradiation were different from those(More)
The time course of recovery in UV- or gamma-irradiated wild-type Schizosaccharomyces pombe has been determined by fractionated dose experiments and by measuring the rate at which the "resistant shoulder" of the survival curve was regained during post-irradiation incubation in growth medium. The kinetics of recovery after UV-irradiation were different from(More)
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