Martina Macková

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The previous article reported on the biochemical characterization of a nitrilase purified from Aspergillus niger K10. The amino acid sequence of this enzyme was recently analyzed by mass spectroscopy which revealed that the N-terminal sequence reported in Fig. 3A (by KB) in the previous article was incorrect. This N-terminal sequence (XAPVLKKYKAAXVNXE),(More)
DNA-based molecular techniques permit the comprehensive determination of microbial diversity but generally do not reveal the relationship between the identity and the function of microorganisms. The first direct molecular technique to enable the linkage of phylogeny with function is DNA-based stable isotope probing (DNA-SIP). Applying this method first(More)
The aim of this work was to determine the ability of rhodococci to transform 3,5-dichloro-4-hydroxybenzonitrile (chloroxynil), 3,5-dibromo-4-hydroxybenzonitrile (bromoxynil), 3,5-diiodo-4-hydroxybenzonitrile (ioxynil) and 2,6-dichlorobenzonitrile (dichlobenil); to identify the products and determine their acute toxicities. Rhodococcus erythropolis A4 and(More)
The chick pineal gland exhibits circadian rhythms in melatonin synthesis under in vivo and in vitro conditions. A daily rhythm of melatonin production was first detectable in pineal glands isolated from chick embryos at embryonic day 16 and incubated under a LD cycle. All pineal glands isolated from 17-day-old and older embryos were rhythmic while no gland(More)
Degradation of chlorobenzoic acids (e.g., products of microbial degradation of PCB) by strains of microorganisms isolated from PCB contaminated soils was assessed. From seven bulk-soil isolates two strains unique in ability to degrade a wider range of chlorobenzoic acids than others were selected, individually and even in a complex mixture of 11 different(More)
Development of a daily rhythmicity in transcription of a gene encoding a rate-limiting enzyme of melatonin biosynthesis, the arylalkylamine-N-acetyltransferase (AA-NAT) was studied by northern blot analysis in pineal glands of 16 and 19-day-old embryos and 1, 4, 8, 11, and 14-day-old chicks. In a parallel experiment, melatonin content in pineal glands and(More)
The pineal cells of chick embryos incubated in vitro exhibited a daily rhythm of melatonin synthesis under a 12:12 light:dark (LD) cycle at the embryonic days 16 and 19. In order to elucidate whether cyclic adenosine monophosphate (cAMP)--a component of the melatonin generating system--is already at work in the embryonic period, we measured the effects of(More)
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