Martina Grmanova

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The “cocktail” of human origin rifampicin-resistant bifidobacteria (RRBs) and RRBs from commercial products was administrated to 9 volunteers aged from 22 to 46 years and the survival ability in gastrointestinal tract of these strains was determined. Bifidobacteria represented 0–8 % of total anaerobes detected in gastrointestinal tract of volunteers before(More)
Resistance or susceptibility of bifidobacteria to lysozyme and growth of bifidobacteria in human milk were tested. Susceptible bifidobacterial strains stopped their growth almost immediately after the addition of lysozyme (400 μg/ml), moderately susceptible strains exhibited reduced growth rate, and growth curves of resistant strains were not affected.(More)
The presence of raffinose series oligosaccharides (RSO) was determined by an enzymic method in three commercially available chicken feed mixtures. All feed mixtures contained RSO at a concentration of 2.1–2.2 %. Soya meal was identified as the exclusive source of RSO. Subsequently, the bifidogenic effect of stachyose (main soya bean RSO) was also assigned(More)
Twenty-five bifidobacteria were isolated from feces of calves. Isolates were identified, and their functional properties and antimicrobial activity were determined. From 10 strains with suitable properties rifampicin-resistant mutants (RRBs) were prepared and mixture of RRBs was administered to 2-d-old calves. These strains were identified by sequencing as(More)
CHARACTERIZATION OF BIFIDOBACTERIA ISOLATED FROM HONEYBEE BY RANDOM DNA AMPLIFICATION M. Grmanova, E. Vlokova, V. Rada, J. Flesar Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Prague, Czech Republic Background: Several methods of DNA analysis have been used to compare and identify bifidobacteria isolated from(More)
Phosphatidylcholine is one of the most frequent phospholipid components of the inner mitochondrial membrane ofNeurospora crassa. Quantitative analysis of phospholipids of the wild strain ofNeurospora crassa and of its twocho mutants showed that these strains did not significantly differ in the content of phosphatidylcholine. Mutants cultivated in a medium(More)
BACKGROUND Myelodysplastic syndrome with isolated chromosome 5q deletion (5q- syndrome) is a clonal stem cell disorder characterized by ineffective hematopoiesis. MicroRNAs (miRNAs) are important regulators of hematopoiesis and their aberrant expression was detected in some clonal hematopoietic disorders. We thus analyzed miRNA expressions in bone marrow(More)
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