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The ATM protein, which is mutated in individuals with ataxia telangi-ectasia (AT), is central to cell cycle checkpoint responses initiated by DNA double-strand breaks (DSBs). ATM's role in DSB repair is currently unclear as is the basis underlying the radiosensitivity of AT cells. We applied immunofluorescence detection of ␥-H2AX nuclear foci and(More)
Immunofluorescence detection of gammaH2AX foci is a widely used tool to quantify the induction and repair of DNA double-strand breaks (DSBs) induced by ionising radiation. We observed that X-irradiation of mammalian cells exposed on glass slides induced twofold higher foci numbers compared to irradiation with gamma-rays. Here, we show that the excess(More)
It is common practice that in teams working in the field of natural sciences all group members manage their primary data in highly individual systems. The consequence of this is that the data are usually lost shortly after the scientific results have been published or that they lose part of their value, as significant background information can no longer be(More)
In this paper, we introduce a scientific format for text-based data files, which facilitates storing and communicating tabular data sets. The so-called Full-Metadata Format builds on the widely used INI-standard and is based on four principles: readable self-documentation, flexible structure, fail-safe compatibility, and searchability. As a consequence, all(More)
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