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The Heawood graph and K 3;3 have the property that all of their 2-factors are Hamilton circuits. We call such graphs 2-factor hamiltonian. We prove that if G is a k-regular bipartite 2-factor hamiltonian graph then either G is a circuit or k ¼ 3: Furthermore, we construct an infinite family of cubic bipartite 2-factor hamiltonian graphs based on the Heawood(More)
Heterologous complementation in yeast has been a successful tool for cloning and characterisation of genes from various organisms. Therefore we constructed conditionally lethal Saccharomyces cerevisiae strains by replacing the endogenous promoter from the genes of interest (glycosyltransferases) by the stringently regulated GAL1-promoter, by a technique(More)
For 3 ≤ k ≤ 20 with k = 4, 8, 12, all the smallest currently known k–regular graphs of girth 5 have the same orders as the girth 5 graphs obtained by the following construction: take a (not necessarily Desarguesian) elliptic semiplane S of order n − 1 where n = k − r for some r ≥ 1; the Levi graph Γ (S) of S is an n–regular graph of girth 6; parallel(More)
We have determined seismic source mechanisms for shallow and intermediate-depth icequake clusters recorded on the glacier Gornergletscher, Switzer-land, during the summers of 2004 and 2006. The selected seismic events are part of a large data set of over 80,000 seismic events acquired with a dense seismic network deployed in order to study the yearly rapid(More)
Isolated nuclei of Saccharomyces cerevisiae were incubated with five restriction nucleases. Out of the twenty-one recognition sequences for these nucleases in the centromere region of chromosome XIV, only five are accessible to cleavage. These sites map 11 by and 74 by to the left and 27 bp, 41 by and 290 by to the right, respectively, of the boundaries of(More)
Although small ubiquitin-like modifier (SUMO) is conjugated to proteins involved in diverse cellular processes, the functional analysis of SUMOylated proteins is often hampered by low levels of specific SUMOylated proteins in the cell. Here we describe a SUMO-conjugating enzyme (Ubc9) fusion-directed SUMOylation (UFDS) system, which allows efficient and(More)
Constitutive and induced protein SUMOylation is involved in the regulation of a variety of cellular processes, such as regulation of gene expression and protein transport, and proceeds mainly in the nucleus of the cell. So far, several hundred SUMOylation targets have been identified, but presumably they represent only a part of the total of proteins which(More)