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A genetically controlled luminescent bacterial reporter assay, the SOS lux test, was developed for rapid detection of environmental genotoxins. The bioassay is based on the recombinant plasmid pPLS-1, which was constructed as a derivative of pBR322, carrying the promoterless luxCDABFE genes of Photobacterium leiognathi downstream of a truncated cda gene(More)
For the b etter part of the 20 th century many large companies have b een focussed on optimizing their mass production process as a way of maximizing their profits. Nowadays, in the existing environment of glob al competitiveness, enhancing the production process remains a significant issue as well. Product configuration b ased on integrated modular product(More)
The effectiveness of heavy ions in inducing the SOS response in E. coli cells has been measured by means of the SOS-chromotest. The SOS induction potency of ionizing radiation increases with increasing LET, to 40-60 keV/microns, reaches a maximum and then decreases at greater LET values. We conclude that the SOS-inducing damages in the case of ionizing(More)
Lambda-prophage induction in repair-deficient and wild-type E. coli strains by heavy ions and gamma-rays has been investigated. The dose dependence of the fraction of induced cells has been measured and its initial slope (lambda-induction potency) determined. The induction by gamma-rays was found to be more efficient in a polA-repair-deficient strain; the(More)
When implementing a product configuration system in a company making complex and highly engineered products, many decisions need to be made in the early phases of the project. This article presents a framework for supporting the initial scoping process and discusses experiences from applying the framework in an engineering company. The framework covers a(More)
Some activation mechanisms of RuDP-carboxylase under illumination are considered. It is assumed on the grounds of experimental data that under illumination thylacoid membranes pass from one phase (I) to another (II). Kinetics of this transition is taken as similar to that of surface phase transition of the first kind. It is assumed that the number of enzyme(More)
The purpose of the work was to study how the number of phage negative colonies on a solid growth medium depended on the concentration of a sensitive bacterial strain. For the system of phage lambda and a sensitive E. coli cell, the number of negative colonies was maximal at a concentration of ca. 4 X 10(8) cells/ml.
A study was made of the influence of the repair genotype on lambda prophage induction by ionizing radiation of different LET in lysogenic E. coli cells. Bacterial strains W3110, P3478, GC244, and 30SO were exposed to gamma-rays and helium ions of 22 keV/microns. Induction of the prophage in GC244 and 30SO strains deficient by lexA and recA genes was either(More)
Configuration systems have widely been applied to efficiently address the customization responsiveness squeeze of companies dealing with Mass Cus-tomization. Over time, several frameworks have been introduced to enable their systematic planning , analyses, development and implementation. Traditional research has thereby either focused on defining modelling(More)