Martin Bonev

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A genetically controlled luminescent bacterial reporter assay, the SOS lux test, was developed for rapid detection of environmental genotoxins. The bioassay is based on the recombinant plasmid pPLS-1, which was constructed as a derivative of pBR322, carrying the promoterless luxCDABFE genes of Photobacterium leiognathi downstream of a truncated cda gene(More)
For the better part of the 20 th century many large companies have been focussed on optimizing their mass production process as a way of maximizing their profits. Nowadays, in the existing environment of global competitiveness, enhancing the production process remains a significant issue as well. Product configuration based on integrated modular product(More)
The effectiveness of heavy ions in inducing the SOS response in E. coli cells has been measured by means of the SOS-chromotest. The SOS induction potency of ionizing radiation increases with increasing LET, to 40-60 keV/microns, reaches a maximum and then decreases at greater LET values. We conclude that the SOS-inducing damages in the case of ionizing(More)
Induction of lambda prophage in lysogenic E. coli cells exposed to ionizing radiation of different LET was studied as a function of dose I(D). Activities of pleiotropic RecA protein were shown to contribute to the shape of the I(D) curve. The experimental data were fitted by the function I(D) = alpha D(1-exp(-D0-1.D]exp(-beta D). Inducibility alpha(More)
When implementing a product configuration system in a company making complex and highly engineered products, many decisions need to be made in the early phases of the project. This article presents a framework for supporting the initial scoping process and discusses experiences from applying the framework in an engineering company. The framework covers a(More)
Lambda-prophage induction in repair-deficient and wild-type E. coli strains by heavy ions and gamma-rays has been investigated. The dose dependence of the fraction of induced cells has been measured and its initial slope (lambda-induction potency) determined. The induction by gamma-rays was found to be more efficient in a polA-repair-deficient strain; the(More)
A study was made of the influence of the repair genotype on lambda prophage induction by ionizing radiation of different LET in lysogenic E. coli cells. Bacterial strains W3110, P3478, GC244, and 30SO were exposed to gamma-rays and helium ions of 22 keV/microns. Induction of the prophage in GC244 and 30SO strains deficient by lexA and recA genes was either(More)
In studying the modifying influence of glycerol and cysteamine of gamma radiation induction of lambda prophage it was shown that dose curves for lambda prophage induced upon irradiation in normal conditions and in the presence of glycerol are those with a maximum. In the presence of cysteamine, the prophage induction is inhibited significantly.