Learn More
We present the design and experimental analysis of a dielectrophoretic trap composed of four microfabricated gold posts excited in a quadrupolar fashion. Using quantitative modeling tools, we have designed these extruded traps to attain strong holding against flow while being constrained by a set of system parameters. The extruded geometry consists of(More)
We have developed a microfabricated device for use in parallel luminescent single-cell assays that can sort populations upon the basis of dynamic functional responses to stimuli. This device is composed of a regular array of noncontact single-cell traps. These traps use dielectrophoresis to stably confine cells and hold them against disrupting fluid flows.(More)
This study investigated the effects of propofol on primary neuronal cultures from rat embryos. Primary cortical neuronal cultures were prepared from Wistar rat embryos (E18). The viability of cells exposed to 0.01, 0.1 or 1 mg/ml propofol for up to 48 h was assessed using a methyltetrazolium assay. In order to evaluate the role of gamma-aminobutyric acid-A(More)
The Alliance for Cellular Signaling is a large-scale collaboration designed to answer global questions about signalling networks. Pathways will be studied intensively in two cells--B lymphocytes (the cells of the immune system) and cardiac myocytes--to facilitate quantitative modelling. One goal is to catalyse complementary research in individual(More)
Dehydroepiandrosterone (DHEA) is a ubiquitous adrenal hormone with immunomodulatory effects such as inhibition of the production of monokines. Whether DHEA itself or the downstream steroids are the immunomodulatory effector hormones in target cells is not known. In this study, we investigated the conversion of DHEA to downstream steroid hormones in target(More)
A single-crystal silicon air turbine supported on gas lubricated bearings has been operated in a controlled and sustained manner at rotational speeds greater then 1 million rpm and power levels approaching 5 W. The device is a second-generation version of the microbearing rig first reported by Lin et al. [1], and is the first micromachine to operate at(More)
—A high-resolution accelerometer with a bulk-micro-machined silicon proof mass and an interferometric position sensor was developed for measuring vibratory accelerations. The inter-ferometer consists of interdigitated fingers that are alternately attached to the proof mass and support substrate. Illuminating the fingers with coherent light generates a(More)
Both cyclooxygenase-1 and -2 are expressed in the spinal cord, and the spinal COX product prostaglandin E(2) (PGE(2)) contributes to the generation of central sensitization upon peripheral inflammation. Vice versa spinal COX inhibition is considered an important mechanism of antihyperalgesic pain treatment. Recently, however, COX-2 was shown to be also(More)