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Probing protein interactions with hydrogen/deuterium exchange and mass spectrometry-a review.
Assessing the functional outcome of protein interactions in structural terms is a goal of structural biology, however most techniques have a limited capacity for making structure-functionExpand
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XRCC4 Protein Interactions with XRCC4-like Factor (XLF) Create an Extended Grooved Scaffold for DNA Ligation and Double Strand Break Repair*♦
The XRCC4-like factor (XLF)-XRCC4 complex is essential for nonhomologous end joining, the major repair pathway for DNA double strand breaks in human cells. Yet, how XLF binds XRCC4 and impactsExpand
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Recombinant immobilized rhizopuspepsin as a new tool for protein digestion in hydrogen/deuterium exchange mass spectrometry.
Hydrogen/deuterium (H/D) exchange coupled to mass spectrometry is nowadays routinely used to probe protein interactions or conformational changes. The method has many advantages, e.g. very low sampleExpand
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Expression and characterization of plant aspartic protease nepenthesin-1 from Nepenthes gracilis.
Carnivorous plants of the genus Nepenthes produce their own aspartic proteases, nepenthesins, to digest prey trapped in their pitchers. Nepenthesins differ significantly in sequence from otherExpand
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Structural and functional characterization of the PNKP–XRCC4–LigIV DNA repair complex
Abstract Non-homologous end joining (NHEJ) repairs DNA double strand breaks in non-cycling eukaryotic cells. NHEJ relies on polynucleotide kinase/phosphatase (PNKP), which generatesExpand
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Neprosin, a Selective Prolyl Endoprotease for Bottom-up Proteomics and Histone Mapping *
Trypsin dominates bottom-up proteomics, but there are reasons to consider alternative enzymes. Improving sequence coverage, exposing proteomic “dark matter,” and clustering post-translationalExpand
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Mass spec studio for integrative structural biology.
The integration of biophysical data from multiple sources is critical for developing accurate structural models of large multiprotein systems and their regulators. Mass spectrometry (MS) can be usedExpand
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Nepenthesin from Monkey Cups for Hydrogen/Deuterium Exchange Mass Spectrometry*
Studies of protein dynamics, structure and interactions using hydrogen/deuterium exchange mass spectrometry (HDX-MS) have sharply increased over the past 5–10 years. The predominant technologyExpand
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Platform Dependencies in Bottom-up Hydrogen/Deuterium Exchange Mass Spectrometry*
Hydrogen-deuterium exchange mass spectrometry is an important method for protein structure-function analysis. The bottom-up approach uses protein digestion to localize deuteration to higherExpand
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Addressing proteolytic efficiency in enzymatic degradation therapy for celiac disease
Celiac disease is triggered by partially digested gluten proteins. Enzyme therapies that complete protein digestion in vivo could support a gluten-free diet, but the barrier to completeness is high.Expand
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