Learn More
DNMT1 is recruited by PCNA and UHRF1 to maintain DNA methylation after replication. UHRF1 recognizes hemimethylated DNA substrates via the SRA domain, but also repressive H3K9me3 histone marks with its TTD. With systematic mutagenesis and functional assays, we could show that chromatin binding further involved UHRF1 PHD binding to unmodified H3R2. These(More)
Any profound comprehension of gene function requires detailed information about the subcellular localization, molecular interactions and spatio-temporal dynamics of gene products. We developed a multifunctional integrase (MIN) tag for rapid and versatile genome engineering that serves not only as a genetic entry site for the Bxb1 integrase but also as a(More)
CC-BY-NC-ND 4.0 International license not peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint (which was. Abstract 1 Single-cell mRNA sequencing (scRNA-seq) allows to profile heterogeneous cell 2 populations, offering exciting possibilities to tackle a variety of biological and medical 3 questions. A(More)
CC-BY-NC-ND 4.0 International license not peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint (which was. Abstract 1 Single-cell mRNA sequencing (scRNA-seq) allows to profile heterogeneous cell 2 populations, offering exciting possibilities to tackle a variety of biological and medical 3 questions. A(More)
  • 1