Marta L. DeDiego

Luis Enjuanes7
Jose L. Nieto-Torres6
7Luis Enjuanes
6Jose L. Nieto-Torres
4Jose A. Regla-Nava
3Carlos Castaño-Rodriguez
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  • Marta L. DeDiego, Jose L. Nieto-Torres, Jose M. Jiménez-Guardeño, Jose A. Regla-Nava, Enrique Álvarez, Juan Carlos Oliveros +4 others
  • 2011
Severe acute respiratory syndrome virus (SARS-CoV) that lacks the envelope (E) gene (rSARS-CoV-ΔE) is attenuated in vivo. To identify factors that contribute to rSARS-CoV-ΔE attenuation, gene expression in cells infected by SARS-CoV with or without E gene was compared. Twenty-five stress response genes were preferentially upregulated during infection in the(More)
  • Ji'An Pan, Xiaoxue Peng, Yajing Gao, Zhilin Li, Xiaolu Lu, Yingzhao Chen +5 others
  • 2008
Analyses of viral protein-protein interactions are an important step to understand viral protein functions and their underlying molecular mechanisms. In this study, we adopted a mammalian two-hybrid system to screen the genome-wide intraviral protein-protein interactions of SARS coronavirus (SARS-CoV) and therefrom revealed a number of novel interactions(More)
  • Fernando Almazán, Marta L. DeDiego, Isabel Sola, Sonia Zuñiga, Jose L. Nieto-Torres, Silvia Marquez-Jurado +2 others
  • 2013
UNLABELLED Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging coronavirus infecting humans that is associated with acute pneumonia, occasional renal failure, and a high mortality rate and is considered a threat to public health. The construction of a full-length infectious cDNA clone of the MERS-CoV genome in a bacterial artificial(More)
  • Jose L. Nieto-Torres, Marta L. DeDiego, Carmina Verdiá-Báguena, Jose M. Jimenez-Guardeño, Jose A. Regla-Nava, Raul Fernandez-Delgado +5 others
  • 2014
Deletion of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) envelope (E) gene attenuates the virus. E gene encodes a small multifunctional protein that possesses ion channel (IC) activity, an important function in virus-host interaction. To test the contribution of E protein IC activity in virus pathogenesis, two recombinant mouse-adapted(More)
  • Jose M. Jimenez-Guardeño, Jose L. Nieto-Torres, Marta L. DeDiego, Jose A. Regla-Nava, Raul Fernandez-Delgado, Carlos Castaño-Rodriguez +1 other
  • 2014
A recombinant severe acute respiratory syndrome coronavirus (SARS-CoV) lacking the envelope (E) protein is attenuated in vivo. Here we report that E protein PDZ-binding motif (PBM), a domain involved in protein-protein interactions, is a major determinant of virulence. Elimination of SARS-CoV E protein PBM by using reverse genetics caused a reduction in the(More)
Systems virology integrates host-directed approaches with molecular profiling to understand viral pathogenesis. Self-contained statistical approaches that combine expression profiles of genes with the available databases defining the genes involved in the pathways (gene-sets) have allowed characterization of predictive gene-signatures associated with(More)
Recently, an avian influenza virus, H5NX subclade, emerged and spread to North America. This subclade has frequently reassorted, leading to multiple novel viruses capable of human infection. Four cases of human infections, three leading to death, have already occurred. Existing vaccine strains do not protect against these new viruses, raising a need(More)
  • Jose M. Jimenez-Guardeño, Jose A. Regla-Nava, Jose L. Nieto-Torres, Marta L. DeDiego, Carlos Castaño-Rodriguez, Raul Fernandez-Delgado +3 others
  • 2015
A SARS-CoV lacking the full-length E gene (SARS-CoV-∆E) was attenuated and an effective vaccine. Here, we show that this mutant virus regained fitness after serial passages in cell culture or in vivo, resulting in the partial duplication of the membrane gene or in the insertion of a new sequence in gene 8a, respectively. The chimeric proteins generated in(More)
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