Marta Calado

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The human immunodeficiency virus replication cycle begins by sequential interactions between viral envelope glycoproteins with CD4 molecule and a member of the seven-transmembrane, G-protein-coupled, receptors' family (coreceptor). In this report we focused on the contribution of CCR8 as alternative coreceptor for HIV-1 and HIV-2 isolates. We found that(More)
Dendritic cells are professional antigen-presenting cells and key elements of both innate and adaptive immunity. Tissues like skin and mucosal epithelium, more exposed to the environment, are particularly rich in dendritic cells. Given that HIV is mainly transmitted through mucosal surfaces, the cellular mechanisms governing the initial interactions between(More)
Human immunodeficiency virus (HIV) entry into susceptible cells involves the interaction between viral envelope glycoproteins with CD4 and a chemokine receptor (coreceptor), namely CCR5 and CXCR4. This interaction has been studied to enable the discovery of a new class of antiretroviral drugs that targets the envelope glycoprotein-coreceptor interaction.(More)
In the last decade many advances have been made in understanding how HIV-1 interacts with macrophages (MØ) and dendritic cells (DC). Based on the notion that HIV-2 is a more attenuate model of infection, we proposed to explore the mechanisms of HIV-2 interaction with MØ and DCs. With this aim, we studied the efficiency of cis-infection of MØ and DCs. A(More)
HIV-2 infection is associated with a slower rate of disease progression with limited impact on the survival of the majority of infected adults, and much lower plasma viral load than HIV-1. In spite of the major differences in viremia, the quantitative assessment of HIV-2 proviral load documented levels similar to those observed in HIV-1 infected(More)
The central proteins for protection against tuberculosis are attributed to interferon-γ, tumor necrosis factor-α, interleukin (IL)-6 and IL-1β, while IL-10 primarily suppresses anti-mycobacterial responses. Several studies found alteration of expression profile of genes involved in anti-mycobacterial responses in macrophages and natural killer (NK) cells(More)
The main goal of this work was to identify molecular signatures in envelope surface glycoprotein that may be correlated with coreceptor usage by different HIV-2 strains. From inspection of aligned HIV-2 sequences we verified that V1/V2 region showed the highest degree of amino acid sequence heterogeneity including polymorphisms in N-linked glycosylation(More)
This work is a continuation of previous studies on phase demixing – salting-out effects – in aqueous nicotine solutions. Thus, pH measurements were performed, allowing a brief analysis of the existing hydrogen bond interactions. Salting-out effects – the related experimental cloud point shifts – provoked by the addition of two inorganic salts, potassium(More)
Human immunodeficiency virus 1 and 2 (HIV-1 and HIV-2) use cellular receptors in distinct ways. Besides a more promiscuous usage of coreceptors by HIV-2 and a more frequent detection of CD4-independent HIV-2 isolates, we have previously identified two HIV-2 isolates (HIV-2MIC97 and HIV-2MJC97) that do not use the two major HIV coreceptors: CCR5 and CXCR4.(More)
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