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The soluble core of catecholamine storage vesicles in the adrenal medulla contains high concentrations of the cations calcium (20 mM) and catecholamine (600 mM). Do these cations interact with the abundant vesicle core anionic proteins, the chromogranins? We investigated the binding of calcium and norepinephrine (NE) to bovine adrenal chromogranins by(More)
BACKGROUND Invasive fungal infections caused by Aspergillus fumigatus represent a great challenge for immunocompromised patients. Pathogen detection is mediated by different receptors, including Toll-like receptors (TLRs), C-type lectins, and pentraxines. However, little is known about their relevance for immature human dendritic cells (iDCs). METHODS The(More)
Chromogranin A is a soluble protein that is stored and released with catecholamines from their secretory vesicles. Its measurement is a probe of exocytotic sympathoadrenal activity, and in plasma it may also be a useful tool in the diagnosis of peptide producing endocrine neoplasms. Because we have found that chromogranin A is elevated in secondary (uremic)(More)
Single nucleotide polymorphisms (SNPs) have been associated with an increased incidence of invasive aspergillosis (IA) after allogeneic stem cell transplantation (allo-SCT). We analyzed 41 patients with proven/probable IA after allo-SCT for an association of SNPs, within the TLR2, TLR4, TLR5, TLR9, and NOD2/CARD15 genes, with susceptibility to IA. The(More)
Chemically modified mRNA is capable of inducing therapeutic levels of protein expression while circumventing the threat of genomic integration often associated with viral vectors. We utilized this novel therapeutic tool to express the regulatory T cell transcription factor, FOXP3, in a time- and site-specific fashion in murine lung, in order to prevent(More)
Patients after allogeneic stem-cell transplantation (alloSCT) have an increased risk for invasive aspergillosis (IA). Here, recipients of an allograft with IA (n=81) or without IA (n=58) were screened for 84 single nucleotide polymorphisms in 18 immune relevant genes. We found 3 markers in chemokine (C-X-C motif) ligand 10 (CXCL10, 4q21, 11,101 C>T, P=.007;(More)
Monocytes play a major role in the cellular defence against Aspergillus fumigatus in immunocompromised patients. To obtain a better understanding of the mechanisms involved in this interaction, phagocytosis and gene expression profiling of human monocytes was carried out after incubation with A. fumigatus resting, swollen and germinating conidia and hyphae(More)
Infections with the opportunistic mold Aspergillus fumigatus show high morbidity and mortality. Risk factors for the development of invasive aspergillosis are neutropenia, T-cell depletion, CD34-selected stem cell products, corticosteroid therapy, and cytomegalovirus infections. Recently, a growing number of defined single nucleotide polymorphisms have been(More)
Promoter variations in Toll-like receptor genes (n = 7) and genes encoding pathogen recognition and virus entry receptors (n = 7) were screened to detect any association with human cytomegalovirus (hCMV) reactivation and disease in patients following allogeneic stem-cell transplantation. Two single nucleotide polymorphisms (rs735240, G>A; rs2287886, C>T) in(More)