Mark S. Rutherford

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Both trimellitic anhydride (TMA), a small molecular weight chemical, and ovalbumin (OVA), a reference protein allergen, cause asthma with eosinophilia. To test the hypothesis that different allergens elicit symptoms of asthma via different effector pathways, gene expression was compared in lungs of Balb/c mice sensitized with either TMA or OVA, followed by(More)
Cryptosporidiosis is caused by an obligate intracellular parasite that has eluded global transcriptional or proteomic analysis of the intracellular developmental stages. The transcript abundance for 3,302 genes (87%) of the Cryptosporidium parvum protein coding genome was elucidated over a 72 hr infection within HCT8 cells using Real Time-PCR. The parasite(More)
Tumor necrosis factor-␣ (TNF-␣), a crucial mediator in sepsis, elicits multiple biologic effects, including intravas-cular thrombosis and circulatory shock. TNF-␣ exerts its bio-logic effects through two distinct cell surface receptors, TNF-R1 and TNF-R2. The pathophysiologic interaction between TNF-␣ and nitric oxide (NO) in glomerular thrombosis caused by(More)
The enteric immune system of swine protects against infectious and noninfectious environmental insults and discriminates ingested nutrients, food, and commensal microflora from pathogenic agents. The molecular and cellular elements of the immune system have been selected over evolutionary time in response to the specific environment of pigs. Thus, models of(More)
The effect of age on several messenger RNAs coding for non-specific immune factors were determined in liver RNA isolated from 6-, 12-, 24-, 29- and 37-month-old male Fischer Rats. The levels of complement factors C3 and C4, complement protein factor B, and alpha 1-acid glycoprotein were determined by dot blot hybridization using cDNA probes. All four mRNAs(More)
The size of the poly(A) segment of poly(A)+RNA extracted from hepatocytes isolated from 4- to 30-month-old rats was determined using polyacrylamide-agarose gel electrophoresis. The average size of the poly(A) segment isolated from newly synthesized poly(A)+RNA was 150 nucleotides, and the average size of the poly(A) segment isolated from the steady state(More)
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