Mark G. Clemens

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Leukocyte motion represents an important component in the innate immune response to infection. Intravital microscopy is a powerful tool as it enables in vivo imaging of leukocyte motion. Under inflammatory conditions, leukocytes may exhibit various motion behaviors, such as flowing, rolling, and adhering. With many leukocytes moving at a wide range of(More)
Intravital microscopy has been used to visualize the microcirculation by imaging fluorescent labeled red blood cells (RBCs). Traditionally, microcirculation has been modeled by computing the mean velocity of a few, randomly selected, manually tracked RBCs. However, this protocol is tedious, time consuming, and subjective with technician related bias. We(More)
BACKGROUND Recurrent ankle injury occurs in 70% of individuals experiencing a lateral ankle sprain. The cause of this high level of recurrence is currently unknown. Researchers have begun to investigate sensorimotor deficits as one possible cause with inconclusive and often conflicting results. The purpose of this study was to further the understanding of(More)
Automated cell tracking using in vivo imagery is difficult, in general, due to the noise inherent in the imaging process, occlusions, varied cell appearance over time, motion of other tissue (distractors), and cells traveling in and out of the image plane. For certain types of cells these problems are exacerbated due to erratic motion patterns. In this(More)
Recent advances in biomedical imaging have enabled the analysis of many different cell types. Learning-based cell detectors tend to be specific to a particular imaging protocol and cell type. For a new dataset, a tedious retraining process is required. In this paper, we present a novel method of training a cell detector on new datasets with minimal effort.(More)
Segmentation of vessels in biomedical images is important as it can provide insight into analysis of vascular morphology, topology and is required for kinetic analysis of flow velocity and vessel permeability. Intravital microscopy is a powerful tool as it enables in vivo imaging of both vasculature and circulating cells. However, the analysis of(More)
BACKGROUND The aim of extracorporeal albumin dialysis (ECAD) is to reduce endogenous toxins accumulating in liver failure. To date, ECAD is conducted mainly with the Molecular Adsorbents Recirculating System (MARS). However, single-pass albumin dialysis (SPAD) has been proposed as an alternative. The aim of this study was to compare the two devices with a(More)
Efficient delivery of short interfering RNAs reflects a prerequisite for the development of RNA interference therapeutics. Here, we describe highly specific nanoparticles, based on near infrared fluorescent polymethine dye-derived targeting moieties coupled to biodegradable polymers. The fluorescent dye, even when coupled to a nanoparticle, mimics a ligand(More)
Nitric oxide (NO) is a pluripotent gaseous free radical that has been identified as an important signaling molecule in virtually every tissue in the body. In the liver, like many other organs, NO has many actions and can be derived from multiple cellular sources. As a result, the exact role of NO in regulating cell or organ function is complex, and(More)
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