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Decreasing oocyte competence with maternal aging is a major factor in human infertility. To investigate the age-dependent molecular changes in a mouse model, we compared the expression profiles of metaphase II oocytes collected from 5- to 6-week-old mice with those collected from 42- to 45-week-old mice using the NIA 22K 60-mer oligo microarray. Among(More)
POU transcription factor Pou5f1 (Oct3/4) is required to maintain ES cells in an undifferentiated state. Here we show that global expression profiling of Oct3/4-manipulated ES cells delineates the downstream target genes of Oct3/4. Combined with data from genome-wide chromatin-immunoprecipitation (ChIP) assays, this analysis identifies not only primary(More)
The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the(More)
Loss of imprinting (LOI) of the insulin-like growth factor II gene (IGF2) is an epigenetic alteration that results in a modest increase in IGF2 expression, and it is present in the normal colonic mucosa of about 30% of patients with colorectal cancer. To investigate its role in intestinal tumorigenesis, we created a mouse model of Igf2 LOI by crossing(More)
Understanding and harnessing cellular potency are fundamental in biology and are also critical to the future therapeutic use of stem cells. Transcriptome analysis of these pluripotent cells is a first step towards such goals. Starting with sources that include oocytes, blastocysts, and embryonic and adult stem cells, we obtained 249,200 high-quality EST(More)
We report that a single growth factor, NM23-H1, enables serial passaging of both human ES and iPS cells in the absence of feeder cells, their conditioned media or bFGF in a fully defined xeno-free media on a novel defined, xeno-free surface. Stem cells cultured in this system show a gene expression pattern indicative of a more "naïve" state than stem cells(More)
which permitsunrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Transcript copy number estimation by microarray <p>An <it>in-situ</it>-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes is presented. Exogenous RNA controls derived from yeast allow(More)
  • M G W A Carter, Sen I E E E Mem, J Y B Z-J Z B Y + M X, M Z-^-± Etc
  • 2005
Synopsis Although the total force on a piece of iron in a magnetic field is calculable by well known methods, the factors governing its distribution over the iron are less well understood. It is shown that the conceptions of iron as an aggregation of dipoles or of current loops are both inadequate for this purpose, but a combination of the two can be used(More)
Stem cells are found in all multicellular organisms, including two broadly defined cell types: embryonic stem cells (ESC) (C. Y. Cheong and T. Lufkin; and N. Lifantseva et al.) that are derived from the inner cell mass of blastocyst-stage embryos and adult stem cells that are present in adult tissues (C. M. Teven et al., R. Chung et al., and A. C. Wilber et(More)
  • R Y O L O G Y B I B L I O G R A P H Y, L Cai, +127 authors Y Zeng
  • 2005
Thyroid hormone controls multiple independent programs required for limb development in Xenopus laevis metamorphosis, expression changes at metamorphosis induced by thyroid hormone in Xenopus laevis tadpoles, Dev. asymmetry of the zebrafish epithalamus guides dorsoventral innvervation of the midbrain target, Lateralization of the vertebrate brain: taking(More)
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