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It is well understood that the key for successful Semantic Web applications depends on the availability of machine understandable meta-data. We describe the Information Grid, a practical approach to the Semantic Web, and show a prototype implementation. Information grid resources span all the data in the organization and all the metadata required to make it(More)
It is well understood that the key for successful Semantic Web applications depends the availability of machine understandable meta-data. In this paper, we describe a practical approach to the Semantic Web called Information Grid. Information grid resources span all the data in the organization and all the metadata required to make it meaningful. This data(More)
The expression of the beta 3 tubulin gene is regulated, at the transcriptional level, by the steroid hormone ecdysone, in Drosophila Kc cells. Using a transient expression assay, we show that 360 bp from the first intron of the beta 3 tubulin gene, associated with the 5' flanking sequences, are essential to confer ecdysone inducibility on a minimum promoter(More)
1. A modified form of the highly specific luciferin-luciferase assay was used to measure the overflow of adenosine 5'-triphosphate (ATP) from the field-stimulated mouse vas deferens in vitro. Precise timing of the stimulation, superfusate collection and assay minimized ATP degradation before assay, offering the opportunity for quantitative studies. 2.(More)
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