Mark C. Pitter

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A heterodyne interference microscope arrangement for full-field imaging is described. The reference and object beams are formed with highly correlated, time-varying laser speckle patterns. The speckle illumination confers a confocal transfer function to the system, and by temporal averaging, the coherence noise that often degrades coherent full-field(More)
A method for the remote detection and identification of liquid chemicals at ranges of tens of meters is presented. The technique uses pulsed indirect photoacoustic spectroscopy in the 10-microm wavelength region. Enhanced sensitivity is brought about by three main system developments: (1) increased laser-pulse energy (150 microJ/pulse), leading to increased(More)
Structured illumination can be employed to extend the lateral resolution of wide-field fluorescence microscopy. Since a structured illumination microscopy image is reconstructed from a series of several acquired images, we develop a modified formulation of the imaging response of the microscope and a probabilistic analysis to assess the resolution(More)
Gold nanostructures of various morphologies, including nanospheres, nanorods, nanoprisms, and thin films, were immobilized on ITO-coated coverslips in order to investigate the response of their scattering to potential. Shifts in the plasmon band obtained by potential-modulated spectroscopic imaging indicated that the voltage sensitivity of the gold(More)
The use of aplanatic solid immersion lenses (ASILs) made of high-refractive-index optical materials provides a route to wide-field high-resolution optical microscopy. Structured illumination microscopy (SIM) can double the spatial bandwidth of a microscope to also achieve high-resolution imaging. We investigate the combination of ASILs and SIM in(More)
Spatial modulation microscopy (SMM) is a technique originally developed for quantitative spectroscopy of individual nano-objects. Here, a parallel implementation of the SMM technique is demonstrated based on a line detector capable of demodulation at kHz frequencies. The capabilities of the imaging system are shown using an array of plasmonic nanoantennas(More)
The stochastic transfer function extends the concept of the conventional transfer function by incorporating noise statistics, thus giving a measure of the signal-to-noise ratio at each spatial frequency. This provides a convenient and standardized metric to assess the trade-off in terms of spatial frequency bandwidth and signal-to-noise ratio for a diverse(More)
Microscopic deformation analysis has been performed using digital image correlation and artificial neural networks (ANNs). Cross-correlations of small image regions before and after deformation contain a peak, the position of which indicates the displacement to pixel accuracy. Subpixel resolution has been achieved here by nonintegral pixel shifting and by(More)
This paper presents a simple, high-resolution, non-fluorescent imaging technique called total internal reflection microscopy (TIRM) and demonstrates its potential application to real-time imaging of live cellular events. In addition, a novel instrument is introduced that combines the simplicity of TIRM with the specificity afforded by dual-colour total(More)